Isolation and Molecular Identification of Aeromonas Wound Infection in Iranian Burn Patients
| Autor: | Tahereh Valadbeygi, Amir Emami, Yalda Malekzadegan, Nahal Hadi, Zhaleh Mahmoodi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Adult Male Cefotaxime Nalidixic acid 030106 microbiology Erythromycin Microbial Sensitivity Tests Iran Microbiology 03 medical and health sciences Immunocompromised Host Young Adult RNA Ribosomal 16S Drug Resistance Bacterial medicine Humans Aeromonas bestiarum Pharmacology biology Coinfection Broth microdilution General Medicine biology.organism_classification Anti-Bacterial Agents 030104 developmental biology Cross-Sectional Studies Aeromonas Wound Infection Molecular Medicine Gentamicin Female Burns Aeromonas veronii medicine.drug |
| Zdroj: | Infectious disorders drug targets. 19(3) |
| ISSN: | 2212-3989 |
| Popis: | Introduction: Aeromonas have recently emerged as opportunistic pathogens and only a few studies are available regarding the isolation of these bacteria from burn wound infections. This study aimed at isolating Aeromonas as an infrequent cause of infection in this group of immunocompromised patients. Methods: A total of 300 samples were collected from the wounds of burn patients hospitalized in Gotbodin Shirazi Burn Center in 2013. The samples were cultured on Aeromonas specific media and then confirmed using standard biochemical tests and 16S rRNA gene amplification and sequencing. The antibiotic susceptibility of the isolates was determined using the disk diffusion and broth microdilution methods. Results: Biochemical tests demonstrated five presumptive samples of Aeromonas, while, molecular testing confirmed only three. All isolates were resistant to ampicillin-sulbactam, erythromycin, oxacillin, and vancomycin. However, they were susceptible to gentamicin, meropenem, nitrofurantoin, chloramphenicol, cephalexin, and cefotaxime. Two Aeromonas veronii isolates were resistant to sulfamethoxazole-trimethoprim and sensitive to nalidixic acid, while Aeromonas bestiarum was sensitive to the former and resistant to the latter antibiotics. Lastly, only one of the A. veronii isolates was found to be resistant to tetracycline. Conclusion: This study is the first to report on the isolation of Aeromonas spp. from burn patients in Iran. Moreover, this is the first report of isolating A. bestiarum from burn wounds. The results of this study confirm earlier findings that the use of molecular methods is essential to accurately identify these bacteria. |
| Databáze: | OpenAIRE |
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