Oxacillin susceptibility testing of coagulase-negative staphylococci using the disk diffusion method and the Vitek GPS-105 card
| Autor: | Baldwin Toye, Wendi Woods, Karam Ramotar, Paul Lem |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Micrococcaceae Microbial Sensitivity Tests Penicillins Muramoylpentapeptide Carboxypeptidase medicine.disease_cause Polymerase Chain Reaction Microbiology Agar dilution Bacterial Proteins Drug Resistance Bacterial Multiplex polymerase chain reaction Staphylococcus epidermidis polycyclic compounds medicine Humans Penicillin-Binding Proteins Agar diffusion test Oxacillin Antibacterial agent biology SCCmec General Medicine Staphylococcal Infections biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Infectious Diseases Hexosyltransferases Peptidyl Transferases bacteria Coagulase Carrier Proteins Staphylococcus |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 42:291-294 |
| ISSN: | 0732-8893 |
| DOI: | 10.1016/s0732-8893(02)00365-6 |
| Popis: | One hundred and ninety-three isolates of coagulase-negative staphylococci (CoNS) were tested against oxacillin by agar dilution, disk diffusion, and Vitek (GPS-105 card), and the presence of the mecA gene determined by multiplex PCR. The results obtained by all testing methods were in agreement for 190 isolates. Two mecA -negative isolates ( S. lugdunensis and S. haemolyticus ) had MICs of ≤0.25 μg/ml by agar dilution and Vitek but were resistant by disk diffusion. One mecA -positive isolate was resistant by Vitek and disk diffusion but had an agar dilution MIC of ≤0.25 μg/ml. For the species of CoNS tested, oxacillin susceptibility results obtained with the Vitek GPS-105 card and disk diffusion correlated well with results obtained by National Committee for Clinical Laboratory Standards agar dilution and with the presence of the mecA gene. |
| Databáze: | OpenAIRE |
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