Neutrophils injure gallbladder interstitial Cajal-like cells in a guinea pig model of acute cholecystitis
Autor: | Hu Qiu, Bao-ping Yu, Meng-Juan Lin, Lei Chen, Zhen-Peng Huang |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Contraction (grammar) Physiology Neutrophils Clinical Biochemistry Cholecystitis Acute Guinea Pigs Cell Communication Immunofluorescence Calcium in biology Contractility Guinea pig 03 medical and health sciences 0302 clinical medicine Immune system medicine Animals Calcium Signaling Telocytes Anoctamin-1 Cells Cultured medicine.diagnostic_test business.industry Gallbladder Cell Biology Coculture Techniques Disease Models Animal Proto-Oncogene Proteins c-kit 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Collagenase Female business medicine.drug Muscle Contraction |
Zdroj: | Journal of cellular physiology. 234(4) |
ISSN: | 1097-4652 |
Popis: | Acute cholecystitis is a common disease with gallbladder dysmotility. Disease pathogenesis involves immune cell infiltration as well as changes in gallbladder interstitial Cajal-like cells (ICLCs). However, it remains unclear if or how the immune cells affect ICLC morphology, density, distribution, and function in gallbladder tissue during acute cholecystitis. In this study, we explored the acute cholecystitis-related alterations in gallbladder ICLCs in a guinea pig model, focusing on the effects of neighboring neutrophils. Adult guinea pigs were randomly divided into four groups (control, 24 hr common bile duct ligation [CBDL], 48-hr CBDL, and antipolymorphonuclear neutrophil [PMN] treated) and analyzed using methylene blue staining and immunofluorescence. Gallbladder contractility was also monitored. To culture gallbladder ICLCs, collagenase digestion was performed on tissue from 10- to 15-day-old guinea pigs. Neutrophils isolated from the peripheral blood of experimental animals 48-hr postsurgery were also cocultured with the gallbladder ICLCs. Intracellular calcium was detected with Fluo-4 AM dye. Our results showed that gallbladder ICLC density significantly declined during acute cholecystitis and was accompanied by shortening of the cellular processes and damage to their network-like structure. However, pretreatment with anti-PMN partially prevented these changes. Gallbladder contraction was also significantly decreased during acute cholecystitis, and this appeared to be mediated by the neutrophils. Moreover, ICLCs cocultured with neutrophils also had shortened and reduced processes and impaired network-like structure formation. Intracellular calcium transient was less sensitive to contraction agonists and inhibitors when cocultured with neutrophils. Taken together, neutrophils greatly affect gallbladder ICLCs and dysmotility during acute cholecystitis. |
Databáze: | OpenAIRE |
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