An enquiry into antileishmanial activity and quantitative analysis of polyhydroxylated steroidal saponins from Solanum paniculatum L. leaves
| Autor: | Alexander B. Valerino-Díaz, Ana C. Zanatta, Wagner Vilegas, Lizandra Guidi Magalhães, Ana Carolina Bolela Bovo Candido, Daylin Gamiotea-Turro, Lourdes Campaner dos Santos |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), University of Franca |
| Rok vydání: | 2020 |
| Předmět: |
UHPLC-ESI-MS
Clinical Biochemistry Antiprotozoal Agents Pharmaceutical Science Solanum 01 natural sciences Analytical Chemistry Inhibitory Concentration 50 Mice Spirostanic saponins Cutaneous leishmaniasis Drug Discovery medicine Animals Quantitative analysis Amastigote IC50 Spectroscopy EC50 Traditional medicine biology 010405 organic chemistry Chemistry Solanumpaniculatum L 010401 analytical chemistry Antileishmanial activity Saponins medicine.disease Solanum paniculatum biology.organism_classification Leishmania In vitro 0104 chemical sciences Plant Leaves Quantitative analysis (chemistry) Brazil Leishmania amazonensis |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1873-264X |
| Popis: | Made available in DSpace on 2021-06-25T11:04:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-11-30 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Solanum paniculatum L. is species whose fruits are widely consumed in Brazil as a tonic beverage with higher content of steroidal saponins. In this work, we developed an analytical method for the quantification of the eight saponins present in the 70 % ethanol extract from the leaves using ultra-high-performance liquid chromatography coupled to mass spectrometry (UHPLC-MS). Besides, the eight spirostanic saponins were screened for in vitro antileishmanial activity against promastigote and amastigote forms of Leishmania (L.) amazonensis. Substances 1, 2 and 3 were found to be the most active compounds, with inhibitory concentration (IC50) values of 8.51 ± 4.38, 10.75 ± 6.85 and 10.45 ± 4.21 μM, respectively, against promastigote forms and effective concentration (EC50) values of >25, 17.73 ± 0.99 and 19.57 ± 0.84 μM, respectively, against amastigote forms. The cytotoxic test with compounds 1–3 evidenced low toxicity in murine macrophage cells, with values above 50 μM at concentration lower than 25 μM. These findings show that saponins 1–3 should be evaluated in further studies for the treatment of cutaneous leishmaniasis. UNESP São Paulo State University Institute of Chemistry, Rua Prof. Francisco Degni, 55 Research Group on Natural Products Center for Research in Sciences and Technology University of Franca, Av. Dr. Armando Salles Oliveira, 201 UNESP São Paulo State University Institute of Biosciences, Praça Infante Dom Henrique, s/n UNESP São Paulo State University Institute of Chemistry, Rua Prof. Francisco Degni, 55 UNESP São Paulo State University Institute of Biosciences, Praça Infante Dom Henrique, s/n CAPES: 187715-1 FAPESP: 2009/52237-9 FAPESP: 2016/21044-4 |
| Databáze: | OpenAIRE |
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