Ongoing Genome Reduction in Mycobacterium ulcerans
| Autor: | Gregor Dernick, Cyrill Mangold, Martin Naegeli, Françoise Portaels, Gerd Pluschke, Timothy P. Stinear, Ulrich Certa, Michel Tessier, Michael Käser, Simona Rondini |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
Buruli ulcer Epidemiology Molecular Sequence Data lcsh:Medicine deletional genomic diversity Genome Genetic diversity lcsh:Infectious and parasitic diseases Evolution Molecular Plasmid Species Specificity medicine Humans lcsh:RC109-216 Fingerprinting Gene Buruli Ulcer Mycobacterium marinum Oligonucleotide Array Sequence Analysis Genetics biology Mycobacterium ulcerans Virulence Research lcsh:R Genetic Variation Bacteriology Genomics Chromosomes Bacterial biology.organism_classification medicine.disease Adaptation Physiological strain variation genomic DNA Infectious Diseases Molecular epidemiology DNA Transposable Elements Geographical distribution microarray Genome Bacterial |
| Zdroj: | Emerging Infectious Diseases Emerging Infectious Diseases, Vol 13, Iss 7, Pp 1008-1008 (2007) |
| ISSN: | 1080-6059 1080-6040 |
| Popis: | M. ulcerans is adapting to a more stable environment. Elucidation of the transmission, epidemiology, and evolution of Mycobacterium ulcerans, the causative agent of Buruli ulcer, is hampered by the striking lack of genetic diversity of this emerging pathogen. However, by using a prototype plasmid-based microarray that covered 10% of the genome, we found multiple genomic DNA deletions among 30 M. ulcerans clinical isolates of diverse geographic origins. Many of the changes appear to have been mediated by insertion sequence (IS) elements IS2404 and IS2606, which have high copy numbers. Classification of the deleted genes according to their biological functions supports the hypothesis that M. ulcerans has recently evolved from the generalist environmental M. marinum to become a niche-adapted specialist. The substantial genomic diversity, along with a prototype microarray that covered a small portion of the genome, suggests that a genome-wide microarray will make available a genetic fingerprinting method with the high resolution required for microepidemiologic studies. |
| Databáze: | OpenAIRE |
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