Generation and characterization of genetically stable heterohybridomas producing foot-and-mouth disease virus-specific porcine monoclonal antibodies
| Autor: | Erica Martel, John G. Neilan, Melia Pisano, José Barrera, Michael Puckette, Lauren Martignette, William Hurtle, Chungwon J. Chung, Mariceny Zurita, Jacob Rutherford |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.drug_class animal diseases Immunology Sus scrofa Biology Monoclonal antibody Virus Epitope Cell Line 03 medical and health sciences Mice 0302 clinical medicine Immune system Antibody Specificity medicine Splenocyte Immunology and Allergy Animals Cloning Molecular B-Lymphocytes Hybridomas Myeloma cell Antibodies Monoclonal Viral Vaccines biology.organism_classification Virology Antibodies Neutralizing 030104 developmental biology Foot-and-Mouth Disease Virus Foot-and-Mouth Disease biology.protein Immunization Foot-and-mouth disease virus Antibody Spleen 030215 immunology |
| Zdroj: | Journal of immunological methods. 487 |
| ISSN: | 1872-7905 |
| Popis: | This report covers the methodology for generation of stable heterohybridoma clones producing Foot-and-mouth disease virus (FMDV) reactive porcine monoclonal antibodies (mAbs). Swine received five inoculations of an inactivated O1 Manisa FMDV vaccine prior to the harvest of splenocytes. Due to the lack of a species-specific hybridoma fusion partner, the Sp2/0 murine myeloma cell line was utilized for the formation of porcine-murine heterohybridoma clones. Twenty-nine FMDV-reactive parental clones were generated. Following sub-cloning and monitoring of reactivity over 20 serial passages, eleven subclones derived from unique parental origins were characterized and are reported herein. This methodology demonstrated the production of porcine mAbs by fusion of porcine splenocytes from immunized pigs with murine myeloma cells to generate heterohybridomas. The porcine immune response may differ from the murine immune response in relation to recognized epitopes. Therefore, application of this methodology may provide valuable resources for swine immunology and enhance the understanding of the mechanisms for antibody based protection from diseases in swine. |
| Databáze: | OpenAIRE |
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