Preparation of group I introns for biochemical studies and crystallization assays by native affinity purification

Autor:	Luis F. Duarte, Quentin Vicens, Anne R. Gooding, Robert T. Batey
Jazyk:	angličtina
Rok vydání:	2009
Předmět:	Transcription Genetic DNA polymerase lcsh:Medicine Biochemistry/Biocatalysis Molecular Biology/RNA Splicing Crystallography X-Ray medicine.disease_cause Polymerase Chain Reaction Chromatography Affinity Exon Affinity chromatography Clostridium botulinum medicine Biochemistry/RNA Structure Group I catalytic intron Nucleotide Biophysics/Biocatalysis lcsh:Science chemistry.chemical_classification Multidisciplinary Base Sequence biology Biochemistry/Structural Genomics lcsh:R Intron Biophysics/Structural Genomics RNA Exons Molecular biology Introns Biophysics/RNA Structure RNA Bacterial chemistry Genes Bacterial biology.protein Nucleic Acid Conformation Biophysics/Experimental Biophysical Methods Electrophoresis Polyacrylamide Gel lcsh:Q Crystallization Research Article
Zdroj:	PLoS ONE, Vol 4, Iss 8, p e6740 (2009) PLoS ONE
ISSN:	1932-6203
Popis:	The study of functional RNAs of various sizes and structures requires efficient methods for their synthesis and purification. Here, 23 group I intron variants ranging in length from 246 to 341 nucleotides -- some containing exons -- were subjected to a native purification technique previously applied only to shorter RNAs (
Databáze:	OpenAIRE
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