A PCR product derived from female DNA with regional localization on the Y chromosome
| Autor: | Alfonso Gomez-Pineda, Carmen López-Fernández, Joaquina de la Torre, Jaime Gosálvez, José L. Fernández, José Luis Díez-Martín, Angel Martínez-Ramírez |
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| Rok vydání: | 2000 |
| Předmět: |
Genetics
Male Satellite DNA Chromosome Mapping General Medicine Biology Y chromosome Molecular biology Polymerase Chain Reaction Chromosome 4 Chromosome 19 Heterochromatin Y Chromosome Constitutive heterochromatin Chromosomes Human Humans Human genome Female Chromosome 21 Molecular Biology Chromosome 22 In Situ Hybridization Fluorescence Biotechnology DNA Primers |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0831-2796 |
| Popis: | A 154-bp PCR product amplified from human female DNA mapped onto the Y chromosome under high-stringency in situ hybridization conditions. The female DNA sequence revealed an 89% homology with the HSDYZ1 sequence. When the same primers were used to amplify male DNA, a 154-bp DNA fragment was also obtained, showing a 98% homology with HSDYZ1. However, although the HSDYZ1 sequence is widely distributed along the long arm of the Y chromosome, both of these particular PCR products are di-regionally localized within this distal block of constitutive heterochromatin. In situ hybridization under lower stringency showed that these 154-bp sequences map both onto the autosomes and the Y chromosome. Overall, this paper shows (i) a new class of DNA sequences shared by the autosomes and the Y chromosome; and (ii) a substructured organization of some DNA repeats within the DYZ1 family that forms a large part of the constitutive heterochromatin of the Y chromosome.Key words: human satellite DNA, satellite 3, DYZ1, Y chromosome. |
| Databáze: | OpenAIRE |
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