Analysis of carbamazepine and its 10,11-epoxide in serum by direct sample injection using surfactant containing eluents and column switching
| Autor: | F V Warren, Stefan Schmitz, Brian A. Bidlingmeyer |
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| Rok vydání: | 1991 |
| Předmět: |
Reproducibility
Chromatography Tandem medicine.diagnostic_test Chemistry Clinical Biochemistry Analytical chemistry Pharmaceutical Science Reproducibility of Results High-performance liquid chromatography Blood proteins Analytical Chemistry Surface-Active Agents Carbamazepine Pulmonary surfactant Therapeutic drug monitoring Phase (matter) Drug Discovery medicine Humans Sample preparation Drug Monitoring Spectroscopy Chromatography Liquid |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 9(10-12) |
| ISSN: | 0731-7085 |
| Popis: | Column switching is used in conjunction with surfactant containing mobile phases and traditional reverse phase LC columns to provide a highly reproducible and accurate analytical procedure for carbamazepine and its 10,11-epoxide in serum. This approach eliminates the tedious sample preparation steps commonly used in the analysis of drugs by HPLC, while providing a high degree of protein removal prior to the final analysis. Various pre-columns were investigated and a pellicular reverse phase column was found suitable for optimum concentration of drugs and removal of serum proteins. A variety of standard reverse phase columns could be used for the analytical separation. The separation of the drugs could be accomplished with a high degree of reproducibility. Tandem pre-column operation was demonstrated to give a sample throughput of 10 h-1. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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