Distribution of coniferin in freeze-fixed stem of Ginkgo biloba L. by cryo-TOF-SIMS/SEM
| Autor: | Ruka Takama, Kazuhiko Fukushima, Katsushi Kuroda, Masato Yoshida, Yasuyuki Matsushita, Dan Aoki, Sachie Yagami, Takuya Akita, Yuto Hanaya |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Spectrometry Mass Secondary Ion Mass spectrometry 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound Botany Microscopy Lignin Multidisciplinary Chromatography biology Plant Stems Ginkgo biloba Ginkgo fungi Cryoelectron Microscopy Xylem food and beverages biology.organism_classification Coniferin 030104 developmental biology chemistry Cinnamates Tracheid Vacuoles Microscopy Electron Scanning 010606 plant biology & botany |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | To clarify the role of coniferin in planta, semi-quantitative cellular distribution of coniferin in quick-frozen Ginkgo biloba L. (ginkgo) was visualized by cryo time-of-flight secondary ion mass spectrometry and scanning electron microscopy (cryo-TOF-SIMS/SEM) analysis. The amount and rough distribution of coniferin were confirmed through quantitative chromatography measurement using serial tangential sections of the freeze-fixed ginkgo stem. The lignification stage of the sample was estimated using microscopic observations. Coniferin distribution visualized at the transverse and radial surfaces of freeze-fixed ginkgo stem suggested that coniferin is stored in the vacuoles and showed good agreement with the assimilation timing of coniferin to lignin in differentiating xylem. Consequently, it is suggested that coniferin is stored in the tracheid cells of differentiating xylem and is a lignin precursor. |
| Databáze: | OpenAIRE |
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