Interleukin-6 Production by Schwann Cells and Induction in Sciatic Nerve Injury
| Autor: | Silver Jon E, Laurel M. Bolin, Eric M. Shooter, A. Neil Verity, Abrams John S |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Nerve Crush
medicine.medical_treatment Cytological Techniques Cell Schwann cell Biology Wounds Nonpenetrating PC12 Cells digestive system Biochemistry Cellular and Molecular Neuroscience Antigens CD Cytokine Receptor gp130 medicine Animals RNA Messenger Autocrine signalling Cells Cultured Membrane Glycoproteins Interleukin-6 Receptors Interleukin Sciatic nerve injury medicine.disease Receptors Interleukin-6 Sciatic Nerve Rats Cell biology medicine.anatomical_structure Cytokine nervous system Immunology Crush injury Cytokines Tumor necrosis factor alpha Schwann Cells Sciatic nerve Signal Transduction |
| Zdroj: | Journal of Neurochemistry. 64:850-858 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1046/j.1471-4159.1995.64020850.x |
| Popis: | Interleukin-6 (IL-6) was produced by the spontaneously immortal Schwann cell clone, iSC, when cocultured with PC12 cells. The iSC cell-derived IL-6 in coculture conditioned media caused the neuronal differentiation of naive PC12 cells and this bioactivity was neutralized by preincubation of conditioned media with antisera to IL-6. Cocultured iSC transcribe IL-6 message as confirmed by northern analysis. Stimuli that induce IL-6 production in the hematopoietic lineage induced transcription and production of IL-6 by iSC cells. Lipopolysaccharide, tumor necrosis factor-alpha, IL-1 alpha, IL-6, and serum withdrawal induced iSC cell IL-6 mRNA. The kinetics of IL-6 production was confirmed in the mouse IL-6-dependent B9 bioassay and that activity could be neutralized with antisera to IL-6. Expression of both the IL-6 receptor and the gp130 signal transduction component by iSC as determined by northern analysis suggests an autocrine regulatory mechanism. The observed iSC production of IL-6 in vitro led to an investigation of the sciatic nerve crush model of Schwann cell activation in vivo. In the initial 12 h after crush injury, IL-6 message is induced. IL-6 mRNA expression was highest distal to the crush injury. Our in vitro data demonstrate that iSC cells produce IL-6 in response to PC12 cell coculture and to stimuli that induce IL-6 production in the hematopoietic lineage. The induction of IL-6 message distal to a crush injury suggests another mechanism by which Schwann cells facilitate peripheral nerve regeneration. |
| Databáze: | OpenAIRE |
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