Influence of the para-aminohippuric acid analysis method on the net hepatic flux of nutrients in lactating cows1
| Autor: | Gonzalo Cantalapiedra-Hijar, Isabelle Ortigues-Marty, A. Isserty-Thomas, J. M. Rodríguez-López, Denys Durand |
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| Přispěvatelé: | Facultad de Ciencias y Tecnologias Quimicas, Universidad de Castilla-La Mancha (UCLM), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), CEA-Direction de l'Energie Nucléaire (CEA-DEN), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, CEA-Direction des Energies (ex-Direction de l'Energie Nucléaire) (CEA-DES (ex-DEN)), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Universidad de Castilla-La Mancha = University of Castilla-La Mancha (UCLM), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
exactitude
030309 nutrition & dietetics [SDV]Life Sciences [q-bio] chemistry.chemical_element Oxygen Absorbance 03 medical and health sciences Flux (metallurgy) Nutrient Genetics medicine Aminohippuric acid acide para-amino-hippurique ComputingMilieux_MISCELLANEOUS 2. Zero hunger 0303 health sciences Chromatography [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health 0402 animal and dairy science circulation sanguine 04 agricultural and veterinary sciences General Medicine Blood flow foie 040201 dairy & animal science Standard curve chemistry Acetylation Animal Science and Zoology bétail [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Food Science medicine.drug |
| Zdroj: | Journal of Animal Science Journal of Animal Science, American Society of Animal Science, 2014, 92 (3), pp.1074-1082. ⟨10.2527/jas.2013-7359⟩ Journal of Animal Science, 2014, 92 (3), pp.1074-1082. ⟨10.2527/jas.2013-7359⟩ |
| ISSN: | 0021-8812 1525-3163 |
| DOI: | 10.2527/jas.2013-7359⟩ |
| Popis: | International audience; Para-aminohippuric acid (pAH) is a marker frequently used to measure plasma or blood flow. In sheep studies, it is recognized that its determination must include a deacetylation step to counteract the hepatic acetylation of pAH. Such a procedure is not of common usage in cattle studies although a recent suggestion of hepatic pAH acetylation in dairy cows may have important consequences for hepatic nutrient fluxes. The aims of this study were to evaluate pAH analytical methods according to international procedures of metrology and confirm hepatic acetylation of pAH in dairy cows. The effect of the matrix used to prepare the standard curve was tested, and the influence of the pAH analytical method on blood flows and subsequent net hepatic fluxes of nutrients was determined. For the first objective, accuracy profiles were established and bias, indicators of precision, and limits of quantification were reported for 2 analytical methods (without and with a pAH deacetylation step) using 2 different standard matrices (water and plasma). Second, the net hepatic flux of different nutrients was determined including or not the deacetylation step and preparing the standard curves in plasma using samples collected from 5 multicatheterized lactating Jersey cows. The choice of the matrix had a significant impact on plasma pAH concentrations as illustrated by accuracy profiles. Water matrix decreased (P < 0.01) the slope, y-intercept, and the absorbance at concentration 0 mg pAH/L of the standard curve in both methods (without and with the deacetylation), supporting that standards prepared in water should not be used to analyze plasma samples. Samples collected on cows confirmed hepatic acetylation of pAH across the liver. Deacetylation, performed using plasma as the standard matrix, increased (P < 0.05) plasma pAH concentrations from 18.4, 26, and 23.5 to 21.4, 28.9, and 27.3 mg/L in the artery, portal vein, and hepatic vein, respectively. Deacetylation decreased the hepatic venous and arterial plasma and blood flows (P < 0.05) by 9 and 55%, respectively, modifying the net hepatic flux of acetate, total amino acid, and oxygen by more than 19% (P < 0.05). In conclusion, our results highlight the importance of including a deacetylation step in the pAH analysis method in cattle studies and of using plasma as standard matrix |
| Databáze: | OpenAIRE |
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