Terbium(III)-thiacalix[4]arene nanosensor for highly sensitive intracellular monitoring of temperature changes within the 303–313 K range

Autor:	Svetlana N. Sudakova, Alexandra D. Voloshina, Kirill A. Sarkanich, Irek R. Nizameev, Dmitry V. Lapaev, Asiya R. Mustafina, Anastasiia S. Sapunova, Alexey P. Dovzhenko, Sergey N. Podyachev, Alberto Vomiero, Amina Daminova, Rustem Zairov, Konstantin A. Petrov
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Science Nanoparticle chemistry.chemical_element Terbium 02 engineering and technology 010402 general chemistry Photochemistry 01 natural sciences Article Metal Colloid Multidisciplinary Quenching (fluorescence) Ligand Chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Physical chemistry visual_art visual_art.visual_art_medium Medicine Materials chemistry Supramolecular chemistry 0210 nano-technology Luminescence Intracellular
Zdroj:	Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) Scientific Reports
ISSN:	2045-2322
Popis:	The work introduces hydrophilic PSS-[Tb2(TCAn)2] nanoparticles to be applied as highly sensitive intracellular temperature nanosensors. The nanoparticles are synthesized by solvent-induced nanoprecipitation of [Tb2(TCAn)2] complexes (TCAn - thiacalix[4]arenes bearing different upper-rim substituents: unsubstituted TCA1, tert-buthyl-substituted TCA2, di- and tetra-brominated TCA3 and TCA4) with the use of polystyrenesulfonate (PSS) as stabilizer. The temperature responsive luminescence behavior of PSS-[Tb2(TCAn)2] within 293–333 K range in water is modulated by reversible changes derived from the back energy transfer from metal to ligand (M* → T1) correlating with the energy gap between the triplet levels of ligands and resonant 5D4 level of Tb3+ ion. The lowering of the triplet level (T1) energies going from TCA1 and TCA2 to their brominated counterparts TCA3 and TCA4 facilitates the back energy transfer. The highest ever reported temperature sensitivity for intracellular temperature nanosensors is obtained for PSS-[Tb2(TCA4)2] (SI = 5.25% K−1), while PSS-[Tb2(TCA3)2] is characterized by a moderate one (SI = 2.96% K−1). The insignificant release of toxic Tb3+ ions from PSS-[Tb2(TCAn)2] within heating/cooling cycle and the low cytotoxicity of the colloids point to their applicability in intracellular temperature monitoring. The cell internalization of PSS-[Tb2(TCAn)2] (n = 3, 4) marks the cell cytoplasm by green Tb3+-luminescence, which exhibits detectable quenching when the cell samples are heated from 303 to 313 K. The colloids hold unprecedented potential for in vivo intracellular monitoring of temperature changes induced by hyperthermia or pathological processes in narrow range of physiological temperatures.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3d43060832c6b645e1b4a8ccbdbe15a https://doaj.org/article/1ae0059bbe1f4b10b3d99b68176735a3 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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