Characterization and expression of different pituitary adenylate cyclase-activating polypeptide/vasoactive intestinal polypeptide receptors in rat ovarian follicles

Autor: Sergio Vaccari, Stefania Latini, Mario Stefanini, Anna Teti, Rita Canipari, Marzia Barberi
Rok vydání: 2006
Předmět:
Cytoplasm
endocrine system
medicine.medical_specialty
Receptors
Vasoactive Intestinal Polypeptide
Type I
Endocrinology
Diabetes and Metabolism
media_common.quotation_subject
Vasoactive intestinal peptide
Fluorescent Antibody Technique
Adenylate kinase
Stimulation
Phosphatidylinositols
Chorionic Gonadotropin
Follicle
Endocrinology
Ovarian Follicle
Internal medicine
Cyclic AMP
medicine
Animals
RNA
Messenger
Rats
Wistar
Receptor
Ovulation
DNA Primers
media_common
Granulosa Cells
Dose-Response Relationship
Drug
Reverse Transcriptase Polymerase Chain Reaction
Chemistry
Stimulation
Chemical
Rats
Apoptosis
Theca
Theca Cells
Oocytes
Pituitary Adenylate Cyclase-Activating Polypeptide
Receptors
Vasoactive Intestinal Peptide
Receptors
Vasoactive Intestinal Peptide
Type II
Calcium
Female
hormones
hormone substitutes
and hormone antagonists
Receptors
Pituitary Adenylate Cyclase-Activating Polypeptide
Type I
Vasoactive Intestinal Peptide
Zdroj: Journal of Endocrinology. 191:287-299
ISSN: 1479-6805
0022-0795
Popis: Pituitary adenylate cyclase-activating polypeptide (PACAP) is a bioactive peptide transiently expressed in preovulatory follicles. PACAP acts by interacting with three types of PACAP receptors. PACAP type I receptor (PAC1-R), which binds specifically to both PACAPs and vasoactive intestinal polypeptide (VIP), although with lower affinity, and two VIP receptors, VPAC1-R and VPAC2-R, which bind to PACAP and VIP with equal affinity. In the present study, we showed the expression of all three receptors in whole ovaries obtained from juvenile and gonadotropin-treated immature rats. A more detailed analysis on cells from preovulatory follicles showed that PAC1-R and VPAC2-R were expressed in granulosa cells, whereas only VIP receptors were expressed in theca/interstitial (TI) cells and fully grown oocytes presented only PAC1-R. The distribution of the VIP receptors was confirmed by immunofluorescence. HCG treatment induced stimulation of PAC1-R in granulosa cells and VPAC2-R in TI cells. The presence of functional PACAP/VIP receptors was also supported by metabolic studies. We further evaluated the presence of PACAP and VIP receptors by testing the effect of these peptides on apoptosis in granulosa cells cultured, isolated or in whole follicles. Treatment of follicles with PACAP and VIP dose-dependently inhibited apoptosis, while only PACAP significantly inhibited isolated granulosa cells. These results demonstrate a different expression of PACAP/VIP receptors in the various follicle compartments and suggest a possible role for PACAP and VIP on granulosa and TI cells, both during follicle development and ovulation.
Databáze: OpenAIRE
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