Increased Toll-Like Receptor 4 Expression on T Cells May Be a Mechanism for Enhanced T cell Response Late After Burn Injury
| Autor: | Bruce A. Cairns, C M. Barnes, Robert Maile, Anthony A. Meyer, Jeffrey A. Frelinger |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
CD4-Positive T-Lymphocytes
Burn injury T cell CD8-Positive T-Lymphocytes Critical Care and Intensive Care Medicine Andrology Mice Immune system T-Lymphocyte Subsets medicine Animals Innate immune system biology business.industry T lymphocyte Acquired immune system Mice Inbred C57BL Toll-Like Receptor 4 Hyaluronan Receptors medicine.anatomical_structure Immunology biology.protein Female Surgery Antibody Burns business Immunologic Memory Spleen CD8 |
| Zdroj: | The Journal of Trauma: Injury, Infection, and Critical Care. 61:293-299 |
| ISSN: | 0022-5282 |
| Popis: | Background: Burn injury is associated with a dynamic T cell response. We have previously reported an enhanced functional T cell response 14 days after burn injury. Toll-like receptors (TLR), primarily expressed on innate immune cells, have recently been identified on certain T cell subsets, including activated and memory T cells. Our hypothesis is that increased TLR4 expression on memory T cells may be a mechanism for enhanced T cell response 14 days after burn injury. Methods: Splenocytes from wild-type C57B1/6 mice were harvested 14 days after a 20% total body surface area (TBSA) scald burn or sham injury. Splenocytes ex vivo were surface stained either with monoclonal anti-CD3, anti-CD4, anti-CD8, or anti-CD44 antibodies or a two-step biotinTLR4 monoclonal antibody-streptavidin-FITC surface stain and results analyzed by flow cytometry. Results: TLR4 expression is successfully detected on CD4 + and CD8 + T cells. TLR4 expression is significantly (p < 0.05) increased on CD4 + T cells and CD8 + T cells 14 days after burn injury. There is a significant (p < 0.05) increase in CD44 hi (memory) CD4 + and CD44 hi (memory) CD8 + T cells 14 days after burn injury and this is associated with a significant (p < 0.05) increase of TLR4 expression in both T cell populations. Conclusions: This study demonstrates for the first time the potential role of TLR4 expression on memory T cells generated late after burn injury. Although further analysis is required, these data reiterate the importance of adaptive immunity and the complexity of the immune response to burn injury. |
| Databáze: | OpenAIRE |
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