Mature Enzymatic Collagen Cross-Links, Hydroxylysylpyridinoline and Lysylpyridinoline, in the Aging Human Vitreous
| Autor: | Johanna M. Snabel, Marielle van Deemter, Roelofje J. van der Worp, Theodorus L. Ponsioen, Rudolf A. Bank, Johanna M. M. Hooymans, Gerrit S. Zijlstra, Leonoor I. Los |
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| Přispěvatelé: | Pharmaceutical Technology and Biopharmacy, Restoring Organ Function by Means of Regenerative Medicine (REGENERATE), Orale Celbiologie (OUD, ACTA), TNO Kwaliteit van Leven |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Senescence
Adult Male medicine.medical_specialty Aging Wet weight Biomedical Research Adolescent 9-FLUORENYLMETHYL CHLOROFORMATE Matrix (biology) DETACHMENT AGE-RELATED-CHANGES Amino acid analysis Internal medicine medicine Humans HYDROXYPYRIDINIUM RESIDUES BODY Amino Acids Child Biology Chromatography High Pressure Liquid Aged chemistry.chemical_classification Aged 80 and over IDENTIFICATION Vitreous Bodies AMINO-ACID-ANALYSIS II PROCOLLAGEN Anatomy Organ Size ARTICULAR-CARTILAGE Middle Aged PERFORMANCE LIQUID-CHROMATOGRAPHY Vitreous Body Endocrinology Enzyme chemistry Ageing Collagen breakdown Child Preschool Female Collagen |
| Zdroj: | Investigative ophthalmology & visual science, 50(3), 1041-1046. ASSOC RESEARCH VISION OPHTHALMOLOGY INC Investigative ophthalmology, 3, 50, 1041-1046 Investigative ophthalmology & visual science, 50(3), 1041-1046. Association for Research in Vision and Ophthalmology Ponsioen, T L, van Deemter, M, Bank, R A, Snabel, J M, Zijlstra, G S, van der Worp, R J, Hooymans, J M M & Los, L I 2009, ' Mature enzymatic collagen cross-links, hydroxylysylpyridinoline and lysylpyridinoline, in the aging human vitreous ', Investigative ophthalmology & visual science, vol. 50, no. 3, pp. 1041-1046 . https://doi.org/10.1167/iovs.08-1714 Investigative Ophthalmology & Visual Science, 50(3), 1041-1046. Association for Research in Vision and Ophthalmology Inc. |
| ISSN: | 0146-0404 1041-1046 |
| DOI: | 10.1167/iovs.08-1714 |
| Popis: | PURPOSE. The vitreous body of the human eye undergoes progressive morphologic changes with aging. Since the enzymatic collagen cross-links hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) are known to be important for the integrity of the collagen matrix, the presence in the vitreous on aging was studied.METHODS. Vitreous bodies (VBs; n = 143) from 119 donors (age 4-80 years; mean +/- SD, 54.3 +/- 17.0 years) were carefully dissected. After weighing and freeze-drying, all samples were analyzed by high performance liquid chromatography. Left and right eyes of 24 donors were compared and, for age-related phenomena, 119 single eyes were used.RESULTS. Within one donor, no significant differences were found between left and right eyes. On aging, VB wet weight (4.42 +/- 0.84 g) accumulates until 35 years and decreases thereafter. Collagen content (0.30 +/- 0.14 mg), HP per triple helix (TH; 0.55 +/- 0.18), and (HP plus LP)/TH (0.61 +/- 0.19) increase until 50 years followed by a decrease, whereas LP/TH (0.057 +/- 0.018) accumulates until 50 years and remains constant thereafter. The ratio between HP and LP (range, 0.42 - 31.0; median, 10.0) is constant over time.CONCLUSIONS. The accumulation of enzymatic collagen cross-links until 50 years is consistent with collagen maturation and possible collagen synthesis in the human vitreous body. The decline of collagen cross-links after 50 years is consistent with collagen breakdown. (Invest Ophthalmol Vis Sci. 2009; 50: 1041-1046) DOI: 10.1167/iovs.08-1714 |
| Databáze: | OpenAIRE |
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