Oxidised LDL (OxLDL) induces production of platelet derived growth factor AA (PDGF AA) from aortic smooth muscle cells

Autor: Valentina Corvino, Sabrina Pagliei, Alessandra Cucina, Valeria Borrelli, Francesco Stipa, Antonio Cavallaro, Antonio V. Sterpetti
Jazyk: angličtina
Rok vydání: 1998
Předmět:
Platelet-derived growth factor
Arteriosclerosis
Thoracic
Aorta
Thoracic
Muscle
Smooth
Vascular
chemistry.chemical_compound
Smooth muscle
Settore BIO/13 - BIOLOGIA APPLICATA
Aorta
Medicine(all)
Platelet-Derived Growth Factor
biology
medicine.diagnostic_test
Blotting
Lipoproteins
LDL
Platelet-Derived Growth Factor-AA
Muscle
lipids (amino acids
peptides
and proteins)
Smooth
Cardiology and Cardiovascular Medicine
Lipoproteins
HDL
Western
Oxidation-Reduction
OxLDL
Platelet-derived growth factor receptor
Cell Division
medicine.medical_specialty
HDL
Lipoproteins
Blotting
Western
High density
LDL
Western blot
Internal medicine
Proliferation rate
Vascular
medicine
Animals
Autocrine signalling
Oxidised low density lipoprotein
business.industry
Atherosclerosis
PDGF AA
Cattle
Endocrinology
chemistry
Immunology
biology.protein
Surgery
business
Popis: Objectives:Elevated concentrations of oxidised low density lipoproteins (OxLDL) are associated with accelerated atherogenesis. The aim of our study was to determine the effect of OxLDL on the proliferation rate and platelet derived growth factor (PDGF) AA production on aortic smooth muscle cells. High density lipoproteins (HDL), which are known to have a protective effect against atherosclerosis, were used as control.Materials and methods:Bovine aortic smooth muscle cells were grown in presence of increased concentrations of OxLDL and HDL and in presence of control medium culture (DMEM). Proliferation rate was assessed by 3H-thymidine uptake. PDGF AA production was determined by ELISA and Western Blot Analysis.Results:OxLDL increased the proliferation rate of aortic smooth muscle cells as compared to DMEM and HDL (p
Databáze: OpenAIRE
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