Highly standardized multicolor femtosecond fiber system for selective microphotomanipulation of deoxyribonucleic acid and chromatin
| Autor: | Christina Czada, Elisa Ferrando-May, Ines Wieser, Michael Schmalz, Alfred Leitenstorfer, Felix Schindler |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microscope Materials science genetic structures Confocal Lasers Solid-State 01 natural sciences law.invention 010309 optics 03 medical and health sciences chemistry.chemical_compound Optics Fiber Bragg grating law Confocal microscopy Fiber laser 0103 physical sciences Fiber Optic Technology Humans ddc:530 DNA Breaks Double-Stranded business.industry DNA Equipment Design Chromatin Atomic and Molecular Physics and Optics 030104 developmental biology chemistry Femtosecond Biophysics sense organs business HeLa Cells |
| Zdroj: | Optics Letters. 43:2877 |
| ISSN: | 1539-4794 0146-9592 |
| Popis: | We present a three-color femtosecond Er/Yb:fiber laser enabling highly specific and standardized nonlinear optical manipulation of live cells. The system simultaneously provides bandwidth-limited 80-fs pulses with identical intensity envelope centered at wavelengths of 515, 775, and 1035 nm in the focus of a confocal microscope. We achieve this goal by combining high-order dispersion control via, for example, chirped fiber Bragg gratings with proper bandwidth management in each nonlinear conversion step. Wavelength-selective and noninterfering induction of deoxyribonucleic acid (DNA) photoproducts and DNA strand breaks, as well as fluorescence photoactivation of a photoactivatable green fluorescent protein (PA-GFP)–histone fusion protein, are demonstrated. The capability to introduce different types of DNA lesions and perform photoswitching experiments in a selective manner is essential for quantitative studies on DNA repair and chromatin dynamics. published |
| Databáze: | OpenAIRE |
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