Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran
| Autor: | Víctor Leandro Papinutti, L. A. Diorio, Flavia Forchiassin |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Dietary Fiber
Chitin Bioengineering Lignin Applied Microbiology and Biotechnology chemistry.chemical_compound Manganese peroxidase Fomes Food science Cellulose Laccase biology Bran Fungi food and beverages Hydrogen-Ion Concentration biology.organism_classification Culture Media Peroxidases Solubility chemistry Biochemistry Fermentation biology.protein Oxidoreductases Biotechnology Peroxidase |
| Zdroj: | Journal of Industrial Microbiology & Biotechnology. 30:157-160 |
| ISSN: | 1476-5535 1367-5435 |
| DOI: | 10.1007/s10295-003-0025-5 |
| Popis: | The aim of this work was to study the growth and production of ligninolytic enzymes by Fomes sclerodermeus using a natural medium based on wheat bran as the principal substrate in a solid-state fermentation. Growth was monitored by measuring the chitin content in the substrate. The maximum rate of growth was observed between days 7 and 18. A 38% total dry-weight loss of the substrate was measured after 28 days of cultivation. Differential hydrolysis of the substrate revealed that cellulose was more extensively degraded than lignin. In the 28-day incubation period, the losses of cellulose and lignin were 38 and 15%, respectively. No lignin peroxidase activity was found in any of the media tested. The maximum manganese-dependent peroxidase activity recorded was 6.3 U g(-1) at 14 days, while the maximum laccase activity was 270 U g(-1) at 28 days post-inoculation. Addition of commonly used inducers such as copper or manganese did not produce a further increase in the enzyme activities, nor did addition of glucose, asparagine, or malt extract. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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