Adhesion of Candida tropicalis to polystyrene and epithelial cell lines: Insights of correlation of the extent of adherent yeast cells among distinct surfaces
| Autor: | C. Caloni, Márcia Cristina Furlaneto, Luciana Furlaneto-Maia, C.M. de Souza, Hugo Felix Perini |
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| Rok vydání: | 2020 |
| Předmět: |
Cell type
Surface Properties Cell Blastoconidium Candida tropicalis HeLa 03 medical and health sciences Chlorocebus aethiops medicine Cell Adhesion Animals Humans Vero Cells 0303 health sciences Microscopy Confocal biology 030306 microbiology Chemistry Epithelial Cells Adhesion biology.organism_classification Molecular biology Infectious Diseases medicine.anatomical_structure Cell culture Vero cell Polystyrenes HeLa Cells |
| Zdroj: | Journal de mycologie medicale. 30(4) |
| ISSN: | 1773-0449 |
| Popis: | Candida tropicalis is an emerging fungal pathogen associated with high mortality. We aimed to compare adherence capability of C. tropicalis to polystyrene and epithelial cell lines (HeLa and Vero), and determine whether adherent blastoconidia is cell-type specific. Blastoconidia adhesion to epithelial cells and polystyrene were determined by crystal violet assay. The percentage of epithelial cells with adhered blastoconidia and the number of adhered blastoconidia per cell line were determined by light microscopy. The correlation between adhesion surfaces was assessed by Pearson's correlation coefficient. The adhesiveness of C. tropicalis to polystyrene was greater than that observed for ephitelial cells. High correlation values (r2 0.9999222, p 0.007941) were found for the adhesion capability between biotic and polystyrene surface for isolates 100.10 (obtained from blood) and 335.07 (obtained from tracheal secretion). The number of adherent blastoconidia per HeLa cell was greater in comparison to that observed for Vero cells (P |
| Databáze: | OpenAIRE |
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