Inhibition of aflatoxin M1 production by bovine hepatocytes after intervention with oltipraz
| Autor: | F.M.A. Woutersen‐van Nijnanten, Johanna Fink-Gremmels, M. E. M. Kuilman, R.F.M. Maas |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Aflatoxin
Metabolite Cell Culture Techniques Food Contamination Thiophenes chemistry.chemical_compound Biotransformation Cytochrome P-450 Enzyme System Oltipraz Animals Anticarcinogenic Agents heterocyclic compounds Mycotoxin General Veterinary biology technology industry and agriculture food and beverages Thiones Metabolism Animal Feed biological factors Enzyme assay Milk chemistry Biochemistry Liver Pyrazines biology.protein Aflatoxin M1 Cattle Drug metabolism |
| Zdroj: | The veterinary quarterly. 22(1) |
| ISSN: | 0165-2176 |
| Popis: | It is well known that cattle ingesting aflatoxin B1 contaminated feed commodities excrete aflatoxin M1 into their milk. As aflatoxin M1 originates from hepatic metabolism, measures to prevent aflatoxin M1 formation need to be directed to either the immobilization of aflatoxin B1 in the gastrointestinal tract or the modification of hepatic metabolism of aflatoxin B1. Here we studied the influence of oltipraz and a second dithiolthione, (1,2) dithiolo (4,3-c)-1,2-dithiole-3,6 dithione (DDD) on bovine hepatic aflatoxin B1 biotransformation. Oltipraz inhibited aflatoxin B1 metabolism as no aflatoxin M1 and no aflatoxin B1-dihydrodiol, the second metabolite found in bovine hepatocytes, was formed. DDD did not significantly inhibit aflatoxin B1 metabolism. It could be demonstrated that the inhibition of aflatoxin B1 metabolism was due to the inhibition of several cytochrome P450 enzyme activities by oltipraz. In contrast, DDD inhibited only ethoxyresorufin O-deethylation activity. These findings suggest a high efficacy of oltipraz in inhibiting aflatoxin M1 contamination of milk from dairy cows exposed to aflatoxin B1 contaminated feeds. |
| Databáze: | OpenAIRE |
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