Distribution of the CAG Triplet Repeat in ATXN1, ATXN3, and CACNA1A Loci in Peruvian Population
| Autor: | Diego Véliz-Otani, Keren Espinoza-Huertas, Mario Cornejo-Olivas, Pilar Mazzetti, Claudia Gonzales-Sáenz, Karina Milla-Neyra, Victoria Marca, Olimpio Ortega, Carolina Cruz-Rodriguez, Jorge Alvarez-Tejada |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male congenital hereditary and neonatal diseases and abnormalities Cerebellar Ataxia Genotype purl.org/pe-repo/ocde/ford#3.02.25 [https] Population Prevalence Biology Frequency 050105 experimental psychology law.invention 03 medical and health sciences 0302 clinical medicine law Peruvian Peru medicine Humans Spinocerebellar Ataxias 0501 psychology and cognitive sciences Triplet repeats Allele education Ataxin-3 Gene Polymerase chain reaction Alleles Ataxin-1 Genetics education.field_of_study Triplet repeat 05 social sciences Machado-Joseph Disease Middle Aged medicine.disease Repressor Proteins Spinocerebellar ataxias Large normal alleles Neurology Spinocerebellar ataxia Female Neurology (clinical) Calcium Channels Trinucleotide Repeat Expansion 030217 neurology & neurosurgery |
| Popis: | Spinocerebellar ataxia subtypes 1, 3, and 6 (SCA1, MJD/SCA3, and SCA6) are among the most prevalent autosomal dominant cerebellar ataxias worldwide, but their relative frequencies in Peru are low. Frequency of large normal (LN) alleles at spinocerebellar ataxia-causative genes has been proposed to be associated with disease prevalence. To investigate the allelic distribution of the CAG repeat in ATXN1, ATXN3, and CACNA1A genes in a Peruvian mestizo population and examine their association with the relative frequency of SCA1, MJD/SCA3, and SCA6 across populations. We genotyped 213 healthy mestizo individuals from Northern Lima, Peru, for ATXN1, ATXN3, and CACNA1A using polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis (PAGE). We compared the frequency of LN alleles and relative disease frequency between populations. We also tested 40 samples for CAT repeat interruptions within the CAG tract of ATXN1. We found no association between disease frequency and population frequency of LN alleles at ATXN1 and ATXN3. All 40 ATXN1 samples tested for CAT interruptions were positive. Frequency of LN alleles at CACNA1A correlates with SCA6 frequency across several populations, but this effect was largely driven by data from a single population. Low frequency of SCA1 and MJD/SCA3 in Peru is not explained by frequency of LN alleles at ATXN1 and ATXN3, respectively. The observed correlation between CACNA1A LN alleles and SCA6 frequency requires further assessment. |
| Databáze: | OpenAIRE |
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