Shift in the function of netrin-1 from axon outgrowth to axon branching in developing cerebral cortical neurons
| Autor: | Masabumi Nagashima, Hideko Matsumoto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Deleted in Colorectal Cancer Neurogenesis Neuronal Outgrowth Receptors Cell Surface Biology Axon collateral branching lcsh:RC321-571 03 medical and health sciences Cellular and Molecular Neuroscience Mice 0302 clinical medicine Pioneer axon Cricetinae Netrin medicine Animals Telodendron Axon outgrowth Axon lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Atmospheric scanning electron microscopy Cells Cultured Cortical development General Neuroscience Axon guidance lcsh:QP351-495 fungi Neuropeptides Netrin-1 Embryonic stem cell Axons lcsh:Neurophysiology and neuropsychology 030104 developmental biology medicine.anatomical_structure nervous system embryonic structures Neuroscience 030217 neurology & neurosurgery Research Article |
| Zdroj: | BMC Neuroscience BMC Neuroscience, Vol 18, Iss 1, Pp 1-12 (2017) |
| ISSN: | 1471-2202 |
| Popis: | Background Netrin-1, a multifunctional axon guidance cue, elicits axon outgrowth via one of its receptors deleted in colorectal cancer (DCC) in several types of neurons, including cerebral cortical neurons of embryonic mice. However, we and others have observed de novo formation of axon branches without axon outgrowth induced by netrin-1 in cortical culture of neonatal hamsters. These previous reports suggested the possibility that netrin-1 function might alter during development, which we here investigated using dissociated culture prepared from cerebral cortices of embryonic mice. Results Imaging analysis revealed netrin-1-induced outgrowth in embryonic day (E) 14 axons and netrin-1-induced branching in E16 axons. Netrin-1-evoked filopodial protrusions, which sprouted on the shafts of E16 axons preceding branch formation, were visualized by a novel method called atmospheric scanning electron microscopy. Treatment with an anti-DCC function-blocking antibody affected both axon outgrowth and branching. Conclusions Morphological analyses suggested a possibility of a shift in the function of netrin-1 in cortical axons during development, from promotion of outgrowth to promotion of branch formation starting with filopodial protrusion. Function-blocking experiments suggested that DCC may contribute not only to axon outgrowth but branching. |
| Databáze: | OpenAIRE |
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