| Popis: |
3,6-Diaminofluoran, the ultimate metabolite of Rhodamine B (tetraethyl-3,6-diaminofluoran) exerted fewer toxic effects in rats than Rhodamine B. The LD50 (intravenous) for Rhodamine B was 89.5 mg/kg compared to and LD50 of 140.0 mg/kg for 3,6-diaminofluoran by the same route. The growth of rats fed 1% Rhodamine B for 90 days was inhibited to a greater extent than that of rats fed 3,6-diaminofluoran at the same dietary level over the same period of time. Liver enlargement, the most significant abnormality at autopsy, occurred to a greater extent in the rats fed Rhodamine B. In vitro incubation studies showed that the metabolism of Rhodamine B occurred in the microsomes of liver cells and duplicated the stepwise de-ethylation process previously observed in the intact animal. Substantially all the orally ingested 3,6-diaminofluoran could be accounted for in the excreta. This indicates that the rat is unable to degrade this metabolite of Rhodamine B. |
