Nitric Oxide Receptor Soluble Guanylyl Cyclase Undergoes Splicing Regulation in Differentiating Human Embryonic Cells
| Autor: | Alexander Y. Kots, Ferid Murad, Emil Martin, Iraida Sharina, Kalpana Mujoo, Vladislav G. Sharin |
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| Rok vydání: | 2011 |
| Předmět: |
inorganic chemicals
Organic Cation Transport Proteins Cellular differentiation Protein subunit Blotting Western Receptors Cytoplasmic and Nuclear Biology Nitric Oxide Cell Line chemistry.chemical_compound Soluble Guanylyl Cyclase Original Research Reports Humans Protein Isoforms heterocyclic compounds Cyclic GMP Cyclic guanosine monophosphate Embryonic Stem Cells Homeodomain Proteins Reverse Transcriptase Polymerase Chain Reaction Alternative splicing Cell Differentiation Nanog Homeobox Protein Cell Biology Hematology Immunohistochemistry Molecular biology Isoenzymes Alternative Splicing Oxidative Stress chemistry Guanylate Cyclase Cell culture RNA splicing cardiovascular system Signal transduction Soluble guanylyl cyclase Octamer Transcription Factor-3 Signal Transduction Developmental Biology |
| Zdroj: | Stem Cells and Development. 20:1287-1293 |
| ISSN: | 1557-8534 1547-3287 |
| Popis: | Nitric oxide (NO), an important mediator molecule in mammalian physiology, initiates a number of signaling mechanisms by activating the enzyme soluble guanylyl cyclase (sGC). Recently, a new role for NO/cyclic guanosine monophosphate signaling in embryonic development and cell differentiation has emerged. The changes in expression of NO synthase isoforms and various sGC subunits has been demonstrated during human and mouse embryonic stem (ES) cells differentiation. Previously, our laboratory demonstrated that nascent α1 sGC transcript undergoes alternative splicing and that expression of α1 sGC splice forms directly affects sGC activity. Expression of sGC splice variants in the process of human ES (hES) cells differentiation has not been investigated. In this report, we demonstrate that α1 sGC undergoes alternative splicing during random hES differentiation for the first time. Our results indicate that C-α1 sGC splice form is expressed at high levels in differentiating cells and its intracellular distribution varies from canonical α1 sGC subunit. Together, our data suggest that alternative splicing of sGC subunits is associated with differentiation of hES cells. |
| Databáze: | OpenAIRE |
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