Characterization of Genes Involved in the Metabolism of α-Galactosides by Lactococcus raffinolactis
| Autor: | Christian Vadeboncoeur, Sylvain Moineau, Isabelle Boucher |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Operon
Molecular Sequence Data Lac repressor Biology Applied Microbiology and Biotechnology chemistry.chemical_compound Galactosides Bacterial Proteins Lactococcus Amino Acid Sequence Raffinose Melibiose Ecology Base Sequence Gene Expression Regulation Bacterial Sequence Analysis DNA Physiology and Biotechnology Galactokinase Leloir pathway chemistry Biochemistry Galactose alpha-Galactosidase Food Science Biotechnology |
| Popis: | Lactococcus raffinolactis , unlike most lactococci, is able to ferment α-galactosides, such as melibiose and raffinose. More than 12 kb of chromosomal DNA from L. raffinolactis ATCC 43920 was sequenced, including the α-galactosidase gene and genes involved in the Leloir pathway of galactose metabolism. These genes are organized into an operon containing aga (α-galactosidase), galK (galactokinase), and galT (galactose 1-phosphate uridylyltransferase). Northern blotting experiments revealed that this operon was induced by galactosides, such as lactose, melibiose, raffinose, and, to a lesser extent, galactose. Similarly, α-galactosidase activity was higher in lactose-, melibiose-, and raffinose-grown cells than in galactose-grown cells. No α-galactosidase activity was detected in glucose-grown cells. The expression of the aga-galKT operon was modulated by a regulator encoded by the upstream gene galR . The product of galR belongs to the LacI/GalR family of transcriptional regulators. In L. lactis , L. raffinolactis GalR acted as a repressor of aga and lowered the enzyme activity by more than 20-fold. We suggest that the expression of the aga operon in lactococci is negatively controlled by GalR and induced by a metabolite derived from the metabolism of galactosides. |
| Databáze: | OpenAIRE |
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