Anti-Candida Targets and Cytotoxicity of Casuarinin Isolated from Plinia cauliflora Leaves in a Bioactivity-Guided Study

Autor: Emerson Izidoro dos Santos, Neil A. R. Gow, Carol A. Munro, Rosemeire Cristina Linhari Rodrigues Pietro, Wagner Vilegas, Tatiana M. Souza-Moreira, Kanya Preechasuth, Juliana A. Severi, Keunsook K. Lee
Přispěvatelé: Universidade Estadual Paulista (Unesp), University of Aberdeen, Alto Universitário
Jazyk: angličtina
Rok vydání: 2013
Předmět:
Hydrolysable tannin
Antifungal Agents
Myrtaceae
Pharmaceutical Science
Plinia cauliflora
ellagitannin
01 natural sciences
Analytical Chemistry
chemistry.chemical_compound
Drug Discovery
Antifungal activity
Candida albicans
Cytotoxicity
Mannan
Candida
chemistry.chemical_classification
0303 health sciences
Ergosterol
biology
Ellagitannin
Cell wall
Candidiasis
Hydrolyzable Tannins
Biochemistry
Chemistry (miscellaneous)
Molecular Medicine
Porosity
food.ingredient
Microbial Sensitivity Tests
Article
Microbiology
lcsh:QD241-441
03 medical and health sciences
food
lcsh:Organic chemistry
Physical and Theoretical Chemistry
Glycoproteins
030304 developmental biology
Organic Chemistry
antifungal activity
biology.organism_classification
Plinia cauliflora (Myrtaceae)
cell wall
0104 chemical sciences
Plant Leaves
010404 medicinal & biomolecular chemistry
chemistry
Casuarinin
Zdroj: Molecules; Volume 18; Issue 7; Pages: 8095-8108
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Molecules, Vol 18, Iss 7, Pp 8095-8108 (2013)
Molecules
ISSN: 1420-3049
DOI: 10.3390/molecules18078095
Popis: Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:29:53Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:32:42Z : No. of bitstreams: 1 2-s2.0-84880795871.pdf: 335742 bytes, checksum: 0852500dbd9c83f1aaf453c928ae8f99 (MD5) Made available in DSpace on 2014-05-27T11:29:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-07-01 In addition to the bio-guided investigation of the antifungal activity of Plinia cauliflora leaves against different Candida species, the major aim of the present study was the search for targets on the fungal cell. The most active antifungal fraction was purified by chromatography and characterized by NMR and mass spectrometry. The antifungal activity was evaluated against five Candida strains according to referenced guidelines. Cytotoxicity against fibroblast cells was determined. The likely targets of Candida albicans cells were assessed through interactions with ergosterol and cell wall composition, porosity and architecture. The chemical major component within the most active antifungal fraction of P. cauliflora leaves identified was the hydrolysable tannin casuarinin. The cytotoxic concentration was higher than the antifungal one. The first indication of plant target on cellular integrity was suggested by the antifungal activity ameliorated when using an osmotic support. The most important target for the tannin fraction studied was suggested by ultrastructural analysis of yeast cell walls revealing a denser mannan outer layer and wall porosity reduced. It is possible to imply that P. cauliflora targeted the C. albicans cell wall inducing some changes in the architecture, notably the outer glycoprotein layer, affecting the cell wall porosity without alteration of the polysaccharide or protein level. © 2013 by the authors. Department of Drugs and Medicines School of Pharmaceutical Sciences UNESP-Univ Estadual Paulista, Rodovia Araraquara-Jau, km 1, Araraquara, 14801-902, São Paulo Department of Microbiology Institute of Medical Sciences University of Aberdeen, Foresterhill, Aberdeen, AB25 2ZD Department of Organic Chemistry Institute of Chemistry UNESP-Univ Estadual Paulista, R. Francisco Degni s/n, Araraquara, 14800-900, São Paulo Department of Pharmacy and Nutrition Centro de Ciências Agrárias Alto Universitário, Guararema, 29500-000, Alegre, ES Department of Drugs and Medicines School of Pharmaceutical Sciences UNESP-Univ Estadual Paulista, Rodovia Araraquara-Jau, km 1, Araraquara, 14801-902, São Paulo Department of Organic Chemistry Institute of Chemistry UNESP-Univ Estadual Paulista, R. Francisco Degni s/n, Araraquara, 14800-900, São Paulo
Databáze: OpenAIRE
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