Adenovirus type 12 E1A down regulates expression of a transgene under control of a major histocompatibility complex class I promoter: evidence for transcriptional control
| Autor: | Johannes L. Bos, Ingeborg Meijer, A. J. Van Der Eb, Aart G. Jochemsen, C.M. de Wit, D Morello |
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| Jazyk: | angličtina |
| Rok vydání: | 1989 |
| Předmět: |
Transcription
Genetic Transgene viruses Immunology Genes MHC Class I Biology medicine.disease_cause Major histocompatibility complex Microbiology Mice Virology MHC class I Gene expression Transcriptional regulation medicine Animals Promoter Regions Genetic Regulation of gene expression MHC Class I Gene Adenovirus Early Proteins Oncogene Proteins Viral Molecular biology Adenoviridae Mice Inbred C57BL Gene Expression Regulation Insect Science biology.protein Research Article |
| Popis: | The E1 region of human adenovirus type 12 (Ad12 E1) represses the expression of major histocompatibility complex (MHC) class I genes in transformed primary rodent cells. Conflicting results have been reported as to whether this E1A-mediated repression occurs at a transcriptional or a posttranscriptional level. In the present study, we show that in Ad12 E1-transformed primary baby mouse kidney cells from transgenic mice harboring the human growth hormone gene under control of an H-2K promoter both the expression of the endogenous MHC class I genes and the expression of the transgene are repressed. This experiment, as well as nuclear run-on analyses performed with single-stranded probes, revealed that Ad12 E1A inhibits MHC class I gene expression by repressing its promoter. |
| Databáze: | OpenAIRE |
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