Selective depletion of Foxp3+ Treg during sensitization phase aggravates experimental allergic airway inflammation
| Autor: | Georg M. N. Behrens, Andrea Hartl, Abdul Mannan Baru, Jayendra Kumar Krishnaswamy, Harald Renz, Tim Sparwasser, Katharina Lahl, Ali Oender Yildirim, Holger Garn, Heinz Fehrenbach |
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| Rok vydání: | 2010 |
| Předmět: |
Ovalbumin
Immunology Population chemical and pharmacologic phenomena Immunoglobulin E T-Lymphocytes Regulatory Lymphocyte Depletion Immune tolerance Mice Immune system T-Lymphocyte Subsets Hypersensitivity medicine Animals Immunology and Allergy education Lung Cells Cultured Sensitization Inflammation education.field_of_study medicine.diagnostic_test biology business.industry FOXP3 Forkhead Transcription Factors hemic and immune systems Allergens respiratory system respiratory tract diseases Mice Inbred C57BL Disease Models Animal Bronchoalveolar lavage medicine.anatomical_structure Immunoglobulin G biology.protein Cytokines Immunization Cytokine secretion business Bronchoalveolar Lavage Fluid |
| Zdroj: | European Journal of Immunology. 40:2259-2266 |
| ISSN: | 0014-2980 |
| DOI: | 10.1002/eji.200939972 |
| Popis: | Recent studies highlight the role of Treg in preventing unnecessary responses to allergens and maintaining functional immune tolerance in the lung. We investigated the role of Treg during the sensitization phase in a murine model of experimental allergic airway inflammation by selectively depleting the Treg population in vivo. DEpletion of REGulatory T cells (DEREG) mice were depleted of Treg by diphtheria toxin injection. Allergic airway inflammation was induced using OVA as a model allergen. Pathology was assessed by scoring for differential cellular infiltration in bronchoalveolar lavage, IgE and IgG1 levels in serum, cytokine secretion analysis of lymphocytes from lung draining lymph nodes and lung histology. Use of DEREG mice allowed us for the first time to track and specifically deplete both CD25(+) and CD25(-) Foxp3(+) Treg, and to analyze their significance in limiting pathology in allergic airway inflammation. We observed that depletion of Treg during the priming phase of an active immune response led to a dramatic exacerbation of allergic airway inflammation in mice, suggesting an essential role played by Treg in regulating immune responses against allergens as early as the sensitization phase via maintenance of functional tolerance. |
| Databáze: | OpenAIRE |
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