A hyperbranched transcription-activated CRISPR-Cas12a signal amplification strategy for sensitive microRNA sensing
Autor: | Xiaoling Liu, Gaoting Wang, Weimin Tian, Chenghui Liu |
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Rok vydání: | 2020 |
Předmět: |
0303 health sciences
Transcription Genetic Computer science Metals and Alloys Biosensing Techniques General Chemistry Computational biology 010402 general chemistry 01 natural sciences Catalysis 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials MicroRNAs 03 medical and health sciences Limit of Detection Transcription (biology) Rolling circle replication microRNA Materials Chemistry Ceramics and Composites CRISPR CRISPR-Cas Systems Signal amplification 030304 developmental biology |
Zdroj: | Chemical Communications. 56:13445-13448 |
ISSN: | 1364-548X 1359-7345 |
DOI: | 10.1039/d0cc06034h |
Popis: | A CRISPR-Cas12a-based strategy is developed for sensitive microRNA sensing, in which hyperbranched rolling circle amplification and transcription are integrated for the activation of the trans-cleavage ability of Cas12a. The integrated triplex signal amplification endows the strategy with a low background and high sensitivity. This design expands the scope of the CRISPR-Cas-based sensing toolbox and shows great potential in biological and biomedical studies. |
Databáze: | OpenAIRE |
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