A hyperbranched transcription-activated CRISPR-Cas12a signal amplification strategy for sensitive microRNA sensing

Autor: Xiaoling Liu, Gaoting Wang, Weimin Tian, Chenghui Liu
Rok vydání: 2020
Předmět:
Zdroj: Chemical Communications. 56:13445-13448
ISSN: 1364-548X
1359-7345
DOI: 10.1039/d0cc06034h
Popis: A CRISPR-Cas12a-based strategy is developed for sensitive microRNA sensing, in which hyperbranched rolling circle amplification and transcription are integrated for the activation of the trans-cleavage ability of Cas12a. The integrated triplex signal amplification endows the strategy with a low background and high sensitivity. This design expands the scope of the CRISPR-Cas-based sensing toolbox and shows great potential in biological and biomedical studies.
Databáze: OpenAIRE