Hepatoprotective Limonoids from Andiroba (Carapa guianensis)
| Autor: | Kaiten Ozeki, Osamu Muraoka, Natsuko Matsuo, Toshio Morikawa, Takashi Kikuchi, Reiko Tanaka, Seiya Miyazawa, Kiyofumi Ninomiya, Takeshi Yamada |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Lipopolysaccharides
Male Carapa guianensis ved/biology.organism_classification_rank.species Pharmacology 01 natural sciences lcsh:Chemistry chemistry.chemical_compound Mice Meliaceae Cytotoxicity lcsh:QH301-705.5 Spectroscopy Cells Cultured Liver injury andiroba hepatoprotective effect General Medicine limonoid structural requirement Computer Science Applications Seeds Tumor necrosis factor alpha Chemical and Drug Induced Liver Injury medicine.drug Limonins Stereochemistry Mice Inbred Strains Flowers Biology 010402 general chemistry Limonoid Nitric Oxide Catalysis Article Nitric oxide Inorganic Chemistry medicine Animals Physical and Theoretical Chemistry Molecular Biology 010405 organic chemistry ved/biology Macrophages Organic Chemistry biology.organism_classification medicine.disease In vitro 0104 chemical sciences chemistry lcsh:Biology (General) lcsh:QD1-999 Hepatocytes |
| Zdroj: | International Journal of Molecular Sciences, Vol 17, Iss 4, p 591 (2016) International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 17; Issue 4; Pages: 591 |
| ISSN: | 1422-0067 |
| Popis: | Three gedunin-type limonoids, gedunin (1), 6α-acetoxygedunin (2), and 7-deacetoxy-7-oxogedunin (3), which were isolated from the seed and flower oils of andiroba (Carapa guianensis Aublet, Meliaceae), exhibited hepatoprotective effects at doses of 25 mg/kg, p.o. against d-galactosamine (d-GalN)/lipopolysaccharide (LPS)-induced liver injury in mice. To characterize the mechanisms of action of 1–3 and clarify the structural requirements for their hepatoprotective effects, 17 related limonoids (1–17) isolated from the seed and/or flower oils of C. guianensis were examined in in vitro studies assessing their effects on (i) d-GalN-induced cytotoxicity in primary cultured mouse hepatocytes, (ii) LPS-induced nitric oxide (NO) production in mouse peritoneal macrophages, and (iii) tumor necrosis factor-α (TNF-α)-induced cytotoxicity in L929 cells. The mechanisms of action of 1–3 are likely to involve the inhibition of LPS-induced macrophage activation and reduced sensitivity of hepatocytes to TNF-α; however, these compounds did not decrease the cytotoxicity caused by d-GalN. In addition, the structural requirements of limonoids (1–17) for inhibition of LPS-induced NO production in mouse peritoneal macrophages and TNF-α-induced cytotoxicity in L929 cells were evaluated. |
| Databáze: | OpenAIRE |
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