Acute hypoxic stress: Effect on blood parameters, antioxidant enzymes, and expression of HIF-1alpha and GLUT-1 genes in largemouth bass (Micropterus salmoides)
| Autor: | Yan Wang, Fu Hongmei, J. Zhou, Shiyong Yang, S.J. Li, Jian-Bo Luo, Xing Ye, Taiming Yan, Song Yang, Qing Xiao, Sun Junlong, Hao Wu, Liulan Zhao |
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| Rok vydání: | 2017 |
| Předmět: |
Fish Proteins
0301 basic medicine Gill Time Factors Antioxidant medicine.medical_treatment Micropterus Aquatic Science Antioxidants Andrology 03 medical and health sciences Gene expression medicine Animals Environmental Chemistry Anaerobiosis chemistry.chemical_classification Regulation of gene expression Glucose Transporter Type 1 biology Aquatic animal 04 agricultural and veterinary sciences General Medicine Anatomy Hypoxia-Inducible Factor 1 alpha Subunit biology.organism_classification Enzyme assay 030104 developmental biology Enzyme Gene Expression Regulation chemistry 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Bass Blood Chemical Analysis |
| Zdroj: | Fish & Shellfish Immunology. 67:449-458 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2017.06.035 |
| Popis: | Dissolved oxygen (DO) plays a crucial role in survival, growth, and normal physiological functions of aquatic organisms. Nevertheless, the mechanisms involved in hypoxic stress and adaptation have not been fully elucidated in Largemouth bass (Micropterus salmoides). To reveal the effect of acute hypoxia on Largemouth bass, we simulated acute hypoxia (DO: 1.2 ± 0.2 mg/L) in the laboratory and analyzed physiological parameters (RBCs, Hb, SOD, CAT, NA+/K+-ATPase, GPx, and MDA) and gene expression (HIF-1alpha and GLUT-1) in Largemouth bass exposed to various durations of acute hypoxia (0, 1, 2, 4, 8, 12, and 24 h). Our results indicated that acute hypoxic exposure significantly increased RBCs but decreased Hb. In addition, antioxidant enzyme activity was enhanced significantly in the liver and muscles at the initial stage of acute hypoxic exposure, but decreased significantly in gills during the entire process of hypoxic exposure. Furthermore, the expression levels of HIF-1alpha and GLUT-1 mRNA were significantly up-regulated in Largemouth bass under acute hypoxic exposure. In conclusion, our study provides a valuable basis for further elucidation of hypoxic adaptation and facilitates husbandry for an economically valuable species. |
| Databáze: | OpenAIRE |
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