Comparison between Different Strategies of Covalent Attachment of DNA to Glass Surfaces to Build DNA Microarrays
| Autor: | Laszlo Hevesi, Laurent Jeanmart, José Remacle, Sandrine Hamels, Nathalie Zammatteo, Pierre Louette, Stéphane Courtois |
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| Rok vydání: | 2000 |
| Předmět: |
Detection limit
Materials science Surface Properties Biophysics Cytomegalovirus Nucleic Acid Hybridization Nanotechnology Sequence (biology) Cell Biology Amplicon Sensitivity and Specificity Biochemistry Molecular biology Fluorescence Kinetics chemistry.chemical_compound chemistry Covalent bond DNA Viral Protein microarray Humans Glass DNA microarray Molecular Biology DNA |
| Zdroj: | Analytical Biochemistry. 280:143-150 |
| ISSN: | 0003-2697 |
| DOI: | 10.1006/abio.2000.4515 |
| Popis: | DNA microarray is a powerful tool allowing simultaneous detection of many different target molecules present in a sample. The efficiency of the array depends mainly on the sequence of the capture probes and the way they are attached to the support. The coupling procedure must be quick, covalent, and reproducible in order to be compatible with automatic spotting devices dispensing tiny drops of liquids on the surface. We compared several coupling strategies currently used to covalently graft DNA onto a glass surface. The results indicate that fixation of aminated DNA to an aldehyde-modified surface is a choice method to build DNA microarrays. Both the coupling procedure and the hybridization efficiency have been optimized. The detection limit of human cytomegalovirus target DNA amplicons on such DNA microarrays has been estimated to be 0.01 nM by fluorescent detection. |
| Databáze: | OpenAIRE |
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