Evaluation of B-cell clonality using the BIOMED-2 PCR method effectively distinguishes cutaneous B-cell lymphoma from benign lymphoid infiltrates
| Autor: | Katie Seo, Daniel A. Arber, Anjali V. Morales, Uma Sundram, Youn H. Kim, Sabine Kohler |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology Lymphoma B-Cell Skin Neoplasms Adolescent Biopsy Cutaneous B-cell lymphoma CBCL Dermatology behavioral disciplines and activities Polymerase Chain Reaction Sensitivity and Specificity Skin Diseases Translocation Genetic Pathology and Forensic Medicine Diagnosis Differential Immunoglobulin kappa-Chains Young Adult medicine Humans B cell Aged Cell Proliferation Aged 80 and over B-Lymphocytes biology Anatomical pathology General Medicine Middle Aged Marginal zone medicine.disease Lymphoma medicine.anatomical_structure Case-Control Studies Immunology biology.protein Female Antibody Clone (B-cell biology) Immunoglobulin Heavy Chains |
| Zdroj: | The American Journal of dermatopathology. 30(5) |
| ISSN: | 1533-0311 |
| Popis: | Primary cutaneous B-cell lymphomas (CBCL) are a diverse group of lymphomas that are limited to the skin at the time of diagnosis. Recently, standardized polymerase chain reaction protocols for immunoglobulin (Ig) rearrangement in nodal malignancies using the BIOMED-2 method have been studied extensively. However, reports of investigations of Ig clonality in CBCL using the BIOMED-2 method have been scant. We hypothesized that clonality detection in CBCL with the BIOMED-2 method could effectively distinguish malignant from benign B-cell-rich infiltrates in the skin. Formalin-fixed tissue samples from 26 patients with CBCL and 23 with benign lymphoid infiltrates were analyzed for Ig clonality using standardized BIOMED-2 polymerase chain reaction protocols. The (14;18) translocation was also assessed. A clone was detected in 22 (85%) of the 26 patients with CBCL [12/15 (80%) marginal zone B-cell lymphoma; 10/11 (91%) follicle center lymphoma] and in 1 (4%) of the 23 patients with benign infiltrates. The (14;18) translocation was present in 3 (12%) of the 26 patients with CBCL [1/15 (7%) marginal zone B-cell lymphoma; 2/11 (18%) follicle center lymphoma]. Our preliminary data indicate that Ig clonality can be detected in formalin-fixed samples of CBCL with meaningful sensitivity (85%) and high specificity (96%) using the BIOMED-2 method. This study forms the basis for further investigating the role of Ig clonality in distinguishing CBCL from benign lymphoid infiltrates that may pose a challenge in morphologic diagnosis. |
| Databáze: | OpenAIRE |
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