Chitosan coating as an antibacterial surface for biomedical applications
| Autor: | Julien Amalric, Mélanie D’Almeida, Brigitte Grosgogeat, Bérangère Toury, François Renaud, Nina Attik, Céline Brunon, Hazem Abouelleil |
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| Přispěvatelé: | Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Science et Surface, Matériaux, ingénierie et science [Villeurbanne] (MATEIS), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Hospices Civils de Lyon (HCL), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Staphylococcus
lcsh:Medicine 02 engineering and technology Pathology and Laboratory Medicine 01 natural sciences Mass Spectrometry Analytical Chemistry Chitosan chemistry.chemical_compound Spectrum Analysis Techniques Secondary Ion Mass Spectrometry Coated Materials Biocompatible Coating antibacterial activity Medicine and Health Sciences biocompatible coated material Staphylococcus Aureus lcsh:Science Titanium Alloys Antiinfective agent Multidisciplinary Antimicrobials Organic Compounds Succinic anhydride Chitosan binding Drugs triethoxysilylpropyl succinic anhydride 021001 nanoscience & nanotechnology Bacterial Pathogens Anti-Bacterial Agents 3. Good health unclassified drug antiinfective agent Chemistry Medical Microbiology Physical Sciences Metallurgy Engineering and Technology Pathogens 0210 nano-technology Antibacterial activity Research Article Chemical Elements Materials Science engineering.material Research and Analysis Methods 010402 general chemistry Microbiology Anhydrides X-ray photoelectron spectroscopy Coatings Microbial Control Alloys succinic anhydride titanium [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials Microbial Pathogens Materials by Attribute Pharmacology Bacteria Surface Treatments Organic Chemistry lcsh:R Organisms Chemical Compounds Biology and Life Sciences X-Ray Photoelectron Spectroscopy 0104 chemical sciences [CHIM.POLY]Chemical Sciences/Polymers Manufacturing Processes chemistry engineering Antibacterials lcsh:Q Time-of-flight mass spectrometry chitosan Electron Beam Spectrum Analysis Techniques Nuclear chemistry |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2017, 12 (12), pp.e0189537. ⟨10.1371/journal.pone.0189537⟩ PLoS ONE, Vol 12, Iss 12, p e0189537 (2017) |
| ISSN: | 1932-6203 |
| Popis: | Background and objectives A current public health issue is preventing post-surgical complications by designing antibacterial implants. To achieve this goal, in this study we evaluated the antibacterial activity of an animal-free chitosan grafted onto a titanium alloy. Methods Animal-free chitosan binding on the substrate was performed by covalent link via a two-step process using TriEthoxySilylPropyl Succinic Anhydride (TESPSA) as the coupling agent. All grafting steps were studied and validated by means of X-ray Photoelectron Spectroscopy (XPS), Time-of-Flight secondary ion mass spectrometry (ToF-SIMS) analyses and Dynamic-mode Secondary Ion Mass Spectrometry (DSIMS). The antibacterial activity against Escherichia coli and Staphylococcus aureus strains of the developed coating was assessed using the number of colony forming units (CFU). Results XPS showed a significant increase in the C and N atomic percentages assigned to the presence of chitosan. A thick layer of polymer deposit was detected by ToF-SIMS and the results obtained by DSIMS measurements are in agreement with ToF-SIMS and XPS analyses and confirms that the coating synthesis was a success. The developed coating was active against both gram negative and gram positive tested bacteria. Conclusion The success of the chitosan immobilization was proven using the surface characterization techniques applied in this study. The coating was found to be effective against Escherichia coli and Staphylococcus aureus strains. © 2017 D'Almeida et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| Databáze: | OpenAIRE |
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