Parechovirus and enteroviruses among young infants with sepsis in Iran
| Autor: | Kambiz Ahmadi Angali, Chiman Karami, Abdolnabi Shabani, Ali Teimoori, Manoochehr Makvandi, Ahmad Shamsizadeh, Roya Pirmoradi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Untranslated region genotype Parechovirus Disease sepsis reverse transcriptase polymerase chain reaction medicine.disease_cause Microbiology Sepsis Genotype medicine enterovirus Genotyping Reverse transcriptase polymerase chain reaction Enterovirus biology business.industry Human parechovirus parechovirus medicine.disease biology.organism_classification Virology QR1-502 Original Article business |
| Zdroj: | Iranian Journal of Microbiology, Vol 13, Iss 3, Pp 312-318 (2021) Iranian Journal of Microbiology |
| ISSN: | 2008-4447 2008-3289 |
| Popis: | Background and Objectives: Human parechoviruses (HPeV) and Human enteroviruses (EV) frequently cause a sepsis-like illness in young infants (younger than three months). Therefore, this study was conducted to determine the frequency of HPeV and EV among the young infants with clinical signs and symptoms of sepsis in Ahvaz city, Iran. Materials and Methods: The blood specimens were collected from 100 (younger than 90 days hospitalized infants) including 54 (56.25%) males and 46 (43.75%) females with clinical signs and symptoms of sepsis-like disease. The RNA was extracted and tested for detection of VP1 region of HPeV and 5 UTR (Untranslated Region) of EV by RT-PCR. The sequences of positive of HPeV were further analyzed to determine HPeV genotyping. Results: 5/100 (5%) of patients including 2/46 (2%) females and 3/54 (3%) males tested positive for HPeV (P=0.85). The analysis of 5 positive VP1 region of HPeV revealed the genotype 1. The analysis of sequencing and phylogenetic tree revealed that the isolated HPeVs were genotype 1. While 38/100 (38%) specimens including 16 (16%) females and 22 (22%) males were tested positive for EV (P=0.68). Conclusion: The frequency of HPeV genotype 1 was 5% among the young infants with sepsis. While frequency of EV was 38% among the young infants with sepsis. This study showed HPeV genotype 1 and EV are dominant in this region. |
| Databáze: | OpenAIRE |
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