Evaluation of column bleed by using an ultraviolet and a charged aerosol detector coupled to a high-temperature liquid chromatographic system
| Autor: | Thekla K. Kiffmeyer, M. Holzhauser, Jochen Tuerk, Thorsten Teutenberg |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Aerosols
Chromatography Hot Temperature Ultraviolet Rays Organic Chemistry Analytical chemistry General Medicine Bleed Hydrogen-Ion Concentration medicine.disease_cause Biochemistry High-performance liquid chromatography Online Systems Charged particle Analytical Chemistry Aerosol Wavelength chemistry.chemical_compound chemistry Chromatography detector Titanium dioxide medicine Ultraviolet Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. A. 1119(1-2) |
| ISSN: | 0021-9673 |
| Popis: | In this study, five different HPLC columns were heated to 200 °C using a homemade heating system which can be operated in temperature programmed mode. The column bleed as an indicator of induced degradation of the stationary phase material was evaluated using a charged aerosol detector (CAD) and an ultraviolet diode array detector (UV-DAD) at different wavelengths. The silica based C-18 stationary phase gave the highest bleed, and the carbon clad titanium dioxide column the lowest bleed. This was independent of both the detection technique and the wavelength. |
| Databáze: | OpenAIRE |
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