PAX7 Targets, CD54, Integrin α9β1, and SDC2, Allow Isolation of Human ESC/iPSC-Derived Myogenic Progenitors
| Autor: | Tania Incitti, Fabrizio Rinaldi, James A. Thomson, Radbod Darabi, Ami Yamamoto, Scott Swanson, Ron Stewart, James Kiley, Rita C.R. Perlingeiro, Alessandro Magli, Jakub Tolar, Sridhar Selvaraj, Ce Yuan |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male muscular dystrophy Cell type Integrins Cellular differentiation medicine.medical_treatment Integrin Induced Pluripotent Stem Cells Gene Expression Muscle Development stem cell therapy General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences Mice SDC2 ChIP sequencing medicine Animals Humans Progenitor cell lcsh:QH301-705.5 muscle regeneration biology PAX7 Transcription Factor Translation (biology) Cell Differentiation RNA sequencing Stem-cell therapy skeletal myogenesis Intercellular Adhesion Molecule-1 Molecular biology PAX7 Cell biology ChIP-sequencing 030104 developmental biology lcsh:Biology (General) biology.protein integrin α9β1 CD54 Syndecan-2 |
| Zdroj: | Cell Reports, Vol 19, Iss 13, Pp 2867-2877 (2017) |
| ISSN: | 2211-1247 |
| Popis: | Summary Pluripotent stem (PS)-cell-derived cell types hold promise for treating degenerative diseases. However, PS cell differentiation is intrinsically heterogeneous; therefore, clinical translation requires the development of practical methods for isolating progenitors from unwanted and potentially teratogenic cells. Muscle-regenerating progenitors can be derived through transient PAX7 expression. To better understand the biology, and to discover potential markers for these cells, here we investigate PAX7 genomic targets and transcriptional changes in human cells undergoing PAX7-mediated myogenic commitment. We identify CD54, integrin α9β1, and Syndecan2 (SDC2) as surface markers on PAX7-induced myogenic progenitors. We show that these markers allow for the isolation of myogenic progenitors using both fluorescent- and CGMP-compatible magnetic-based sorting technologies and that CD54+α9β1+SDC2+ cells contribute to long-term muscle regeneration in vivo. These findings represent a critical step toward enabling the translation of PS-cell-based therapies for muscle diseases. |
| Databáze: | OpenAIRE |
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