Preclinical Model of Hind Limb Ischemia in Diabetic Rabbits
| Autor: | Aaron B. Baker, Patricia Felli, Gretchen Howe, Varsha Karanam, Andrew D. Sligar, Julia Goldman, Richard W. Smalling |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty medicine.medical_treatment General Chemical Engineering Ischemia Hyperlipidemias Femoral artery General Biochemistry Genetics and Molecular Biology Diabetes Mellitus Experimental Atherectomy 03 medical and health sciences 0302 clinical medicine Restenosis medicine.artery Internal medicine Angioplasty medicine Animals cardiovascular diseases Neovascularization Pathologic General Immunology and Microbiology Vascular disease business.industry General Neuroscience Critical limb ischemia medicine.disease Peripheral Hindlimb Femoral Artery Disease Models Animal 030104 developmental biology 030220 oncology & carcinogenesis Cardiology Rabbits medicine.symptom business |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| Popis: | Peripheral vascular disease is a widespread clinical problem that affects millions of patients worldwide. A major consequence of peripheral vascular disease is the development of ischemia. In severe cases, patients can develop critical limb ischemia in which they experience constant pain and an increased risk of limb amputation. Current therapies for peripheral ischemia include bypass surgery or percutaneous interventions such as angioplasty with stenting or atherectomy to restore blood flow. However, these treatments often fail to the continued progression of vascular disease or restenosis or are contraindicated due to the overall poor health of the patient. A promising potential approach to treat peripheral ischemia involves the induction of therapeutic neovascularization to allow the patient to develop collateral vasculature. This newly formed network alleviates peripheral ischemia by restoring perfusion to the affected area. The most frequently employed preclinical model for peripheral ischemia utilizes the creation of hind limb ischemia in healthy rabbits through femoral artery ligation. In the past, however, there has been a strong disconnect between the success of preclinical studies and the failure of clinical trials regarding treatments for peripheral ischemia. Healthy animals typically have robust vascular regeneration in response to surgically induced ischemia, in contrast to the reduced vascularity and regeneration in patients with chronic peripheral ischemia. Here, we describe an optimized animal model for peripheral ischemia in rabbits that includes hyperlipidemia and diabetes. This model has reduced collateral formation and blood pressure recovery in comparison to a model with a higher cholesterol diet. Thus, the model may provide better correlation with human patients with compromised angiogenesis from the common co-morbidities that accompany peripheral vascular disease. |
| Databáze: | OpenAIRE |
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