A gene cluster from Streptomyces galilaeus involved in glycosylation of aclarubicin
| Autor: | Kaj Räty, Kristiina Ylihonko, J. Hakala, Pekka Mäntsälä, Tero Kunnari |
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| Rok vydání: | 2000 |
| Předmět: |
Glycosylation
Molecular Sequence Data Mutant Hybrid Cells Biology Plasmid Bacterial Proteins Gene cluster Genetics medicine Anthracyclines Gene Silencing Cloning Molecular Aclarubicin Molecular Biology Gene Antibiotics Antineoplastic Activator (genetics) Genetic Complementation Test Glycosyltransferases Methyltransferases Nucleotidyltransferases Streptomyces Complementation Subcloning Biochemistry Multigene Family Mutation Plasmids medicine.drug |
| Zdroj: | Molecular and General Genetics MGG. 264:164-172 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/s004380000306 |
| Popis: | We have cloned and characterized a gene cluster for anthracycline biosynthesis from Streptomyces galilaeus. This cluster, 15-kb long, includes eight genes involved in the deoxyhexose biosynthesis pathway, a gene for a glycosyltransferase and one for an activator, as well as two genes involved in aglycone biosynthesis. Gene disruption targeted to the activator gene blocked production of aclacinomycins in S. galilaeus. Plasmid pSgs4, containing genes for a glycosyltransferase (aknS), an aminomethylase (aknX), a glucose-1-phosphate thymidylyltransferase (akn Y) and two genes for unidentified glycosylation functions (aknT and aknV), restored the production of aclacinomycins in the S. galilaeus mutants H063, which accumulates aklavinone, and H054, which produces aklavinone with rhodinose and deoxyfucose residues. Furthermore, pSgs4 directed the production of L-rhamnosyl-epsilon-rhodomycinone and L-daunosaminyl-epsilon-rhodomycinone in S. peucetius strains that produce epsilon-rhodomycinone endogenously. Subcloning of the gene cluster was carried out in order to further define the genes that are responsible for complementation and hybrid anthracycline generation. |
| Databáze: | OpenAIRE |
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