Impact of delayed and prolonged fixation on the evaluation of immunohistochemical staining on lung carcinoma resection specimen
| Autor: | Iva Brcic, Luka Brcic, Rosita Kammler, Irene Sansano, Thomas Geiger, Birgit I. Lissenberg-Witte, Matyas Bendek, Atilio Navarro Gonzales, Erik Thunnissen, H. Ibrahim Korkmaz, Rolf A. Stahel, Maartje van Seijen |
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| Přispěvatelé: | University of Zurich, Thunnissen, Erik, Pathology, Other Research, Epidemiology and Data Science, APH - Methodology, Amsterdam Movement Sciences - Restoration and Development, CCA - Imaging and biomarkers |
| Rok vydání: | 2019 |
| Předmět: |
Lung adenocarcinoma
0301 basic medicine Pathology medicine.medical_specialty Lung Neoplasms Tissue Fixation 610 Medicine & health Pre-analytical Pathology and Forensic Medicine 1307 Cell Biology 03 medical and health sciences 0302 clinical medicine Carcinoma Non-Small-Cell Lung Keratin 1312 Molecular Biology Biomarkers Tumor Carcinoma medicine Humans Lung cancer Molecular Biology Fixation (histology) chemistry.chemical_classification Tissue microarray Lung Staining and Labeling business.industry Cell Biology General Medicine medicine.disease Fixation Immunohistochemistry Staining 2734 Pathology and Forensic Medicine 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis 10032 Clinic for Oncology and Hematology Original Article business |
| Zdroj: | van Seijen, M, Brcic, L, Gonzales, A N, Sansano, I, Bendek, M, Brcic, I, Lissenberg-Witte, B, Korkmaz, H I, Geiger, T, Kammler, R, Stahel, R, Thunnissen, E & On behalf of ETOP 2019, ' Impact of delayed and prolonged fixation on the evaluation of immunohistochemical staining on lung carcinoma resection specimen ', Virchows Archiv, vol. 475, no. 2, pp. 191-199 . https://doi.org/10.1007/s00428-019-02595-9 Virchows Archiv Virchows Archiv, 475(2), 191-199. Springer Verlag |
| ISSN: | 1432-2307 0945-6317 |
| Popis: | Pre-analytical factors, such as fixation time, influence morphology of diagnostic and predictive immunohistochemical staining, which are increasingly used in the evaluation of lung cancer. Our aim was to investigate if variations in fixation time influence the outcome of immunohistochemical staining in lung cancer. From lung resections, specimen with tumor size bigger than 4 cm, 10 samples were obtained: 2 were put through the standard fixation protocol, 5 through the delayed, and 3 through the prolonged fixation protocol. After paraffin embedding, tissue microarrays (TMAs) were made. They were stained with 20 antibodies and scored for quality and intensity of staining. Samples with delay in fixation showed loss of TMA cores on glass slides and deterioration of tissue quality leading to reduction in the expression of CK 7, Keratin MNF116, CAM 5.2, CK 5/6, TTF-1, C-MET, Napsin A, D2-40, and PD-L1. Prolonged fixation had no influence on the performance of immunohistochemical stains. Delay of fixation negatively affects the expression of different immunohistochemical markers, influencing diagnostic (cytokeratins) and predictive (PD-L1) testing. These results emphasize the need for adequate fixation of resection specimen. Electronic supplementary material The online version of this article (10.1007/s00428-019-02595-9) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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