Cord Blood G 0 CD34 + Cells Have A Thousand‐Fold Higher Capacity For Generating Progenitors In Vitro Than G 1 CD34 + Cells
| Autor: | Clare M. Heyworth, Erika A. de Wynter, Yvonne J. Summers, Nydia G Testa, James Chang |
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| Rok vydání: | 2001 |
| Předmět: |
Time Factors
Population CD34 Antigens CD34 Cell Count Stem cell factor Biology Resting Phase Cell Cycle Humans Progenitor cell education education.field_of_study Cell Cycle G1 Phase Cell Biology Cell cycle Fetal Blood Flow Cytometry Hematopoietic Stem Cells Molecular biology In vitro Cord blood Immunology Molecular Medicine Stem cell Cell Division Developmental Biology |
| Zdroj: | STEM CELLS. 19:505-513 |
| ISSN: | 1549-4918 1066-5099 |
| DOI: | 10.1634/stemcells.19-6-505 |
| Popis: | We examined the functional differences between G(0) and G(1) cord blood CD34+ cells for up to 24 weeks in serum-free suspension culture, containing Flt-3 ligand, thrombopoietin and stem cell factor. By week 24, there is more than a 1,000-fold difference in granulocyte, macrophage-colony-forming cells (GM-CFC) cumulative production between the two populations, with cultures initiated from G(0) demonstrating an amplification of 1.1 x 10(5)-1.8 x 10(6) of GM-CFC compared to 45-2.7 x 10(3) for the G(1) cells. Cells from the initial G(0) population are able to produce about 250-fold higher numbers of BFU-E than those from G(1) which translates to 3 x 10(3)-1.1 x 10(5)-fold expansion and 25-390-fold expansion for G(0) and G(1), respectively. This amplification of the progenitor cells is reflected in finding that a greater proportion of the progeny of the G(0) population are CD34+, resulting in a 600-fold expansion of CD34+ cells at week 8. As in other in vitro systems, total cell expansion is less discriminatory of stem cell behavior than progenitor cells, and there is no significant difference in total cell numbers between G(0) and G(1) cultures with a mean fold expansion of 2 x 10(7) at 24 weeks. |
| Databáze: | OpenAIRE |
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