New vessel formation in peritumoral area of squamous cell carcinoma of the head and neck
| Autor: | Genya Yanagida, David Hollemann, Beate M. Rüger, Michael B. Fischer, Csilla Neuchrist |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
CD31 Pathology medicine.medical_specialty Endothelium Angiogenesis CD34 Antigens CD34 Neovascularization Antigens CD medicine Humans AC133 Antigen Aged Glycoproteins Neovascularization Pathologic business.industry Endothelial Cells Middle Aged medicine.disease Vascular Endothelial Growth Factor Receptor-2 Head and neck squamous-cell carcinoma Platelet Endothelial Cell Adhesion Molecule-1 Endothelial stem cell medicine.anatomical_structure Otorhinolaryngology Head and Neck Neoplasms Antigens Surface Carcinoma Squamous Cell cardiovascular system RNA Endothelium Vascular Pericyte medicine.symptom Peptides Pericytes business |
| Zdroj: | Head & Neck. 34:813-820 |
| ISSN: | 1043-3074 |
| DOI: | 10.1002/hed.21814 |
| Popis: | Background This study was conducted to investigate the dynamic process of new vessel formation, fundamental for tumor growth and metastasis, in head and neck squamous cell carcinoma (HNSCC). Methods We used immunohistochemistry, confocal laser-scanning microscopy, and reverse transcriptase–polymerase chain reaction to study endothelial cell and concomitant pericyte development with markers CD133, CD34, VEGFR-2, CD31, vWF, and STRO-1 in tumor and peritumoral tissues of 18 patients with HNSCC. Results Highly compressed and structurally abnormal vessels with barely any activity of new vessel formation were found in tumor tissue, whereas the adjacent peritumoral tissue vessels showed a normal architecture with tight endothelial cell–pericyte interaction and a high activity of angiogenesis. Endothelial precursor cells expressing CD133/VEGFR-2 could be incorporated into these newly formed vessels, forming cell clusters from which a thin endothelial lining could emanate. Conclusions These data show a high activity of new vessel formation in the peritumoral stroma of HNSCC, with endothelial precursor cells being incorporated into these structures. © 2011 Wiley Periodicals, Inc. Head Neck, 2011 |
| Databáze: | OpenAIRE |
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