A sensitive LC-MS/MS method for simultaneous determination of amygdalin and paeoniflorin in human plasma and its application
| Autor: | Xiaobing Li, Hua He, Li Ding, Fuguo Shi, Lingye Liu, Pan Gu |
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| Rok vydání: | 2013 |
| Předmět: |
Analyte
Chromatography Chemistry Amygdalin Clinical Biochemistry Relative standard deviation Analytical chemistry Pharmaceutical Science Pilot Projects Paeoniflorin Analytical Chemistry Adduct chemistry.chemical_compound Pharmacokinetics Glucosides Human plasma Tandem Mass Spectrometry Drug Discovery Lc ms ms Monoterpenes Humans Spectroscopy Chromatography Liquid Drugs Chinese Herbal |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 92 |
| ISSN: | 1873-264X |
| Popis: | A simple and sensitive HPLC-MS/MS method was developed and fully validated for the simultaneous determination of amygdalin (AD) and paeoniflorin (PF) in human plasma. For both analytes, the method exhibited high sensitivity (LLOQs of 0.6ng/mL) by selecting the ammonium adduct ions ([M+NH4](+)) as the precursor ions and good linearity over the concentration range of 0.6-2000ng/mL with the correlation coefficients>0.9972. The intra- and inter-day precision was lower than 10% in relation to relative standard deviation, while accuracy was within ±2.3% in terms of relative error for both analytes. The developed method was successfully applied to a pilot pharmacokinetic study of AD and PF in healthy volunteers after intravenous infusion administration of Huoxue-Tongluo lyophilized powder for injection. |
| Databáze: | OpenAIRE |
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