Circulating Structurally Related (-)-Epicatechin Metabolite Species and Levels after Sustained Intake of a Cocoa Powder High in Polyphenols Are Comparable to Those Achieved after a Single Dose
| Autor: | Paloma K Barrera-Reyes, Elizabeth M. Tejero, Laure Poquet, Martin Kussmann, Josué Cortés-Fernández de Lara, Irma Silva-Zolezzi, Karine Redeuil |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Adolescent Metabolite Biological Availability antioxidant capacity Catechin Article chemistry.chemical_compound Eating Young Adult Tandem Mass Spectrometry healthy adults Humans TX341-641 Food science Chocolate Chromatography High Pressure Liquid polyphenols Nutrition and Dietetics Cross-Over Studies Chemistry Nutrition. Foods and food supply food and beverages epicatechin Healthy Volunteers quantification Bioavailability Antioxidant capacity Polyphenol cocoa Dietary Supplements Female epicatechin metabolites Metabolic profile Cocoa polyphenols Food Science |
| Zdroj: | Nutrients, Vol 13, Iss 3829, p 3829 (2021) Nutrients Volume 13 Issue 11 |
| ISSN: | 2072-6643 |
| Popis: | Background: While the bioavailability of cocoa polyphenols, particularly of the monomer (-)-epicatechin, has been investigated after a single-dose intake, the effect of sustained cocoa consumption on the metabolic profile of the structurally related (-)-epicatechin metabolites (SREMs) has not been investigated. Methods: A randomized, controlled crossover clinical trial in healthy young adults (18–40 year) was conducted to evaluate SREMs after consumption of a single-dose and after daily consumption of 1.3 g of polyphenol-rich cocoa powder for 28 days. The circulating SREMs were measured by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Results: Twenty subjects (eleven males and nine females) were enrolled. The SREMs concentrations increased to 1741 ± 337 nM after a single-dose and to 1445 ± 270 nM after sustained supplementation. Sulfate conjugates showed higher levels in females (p < 0.05). The epicatechin-3′-glucuronide (E3′G) and epicatechin-3′-sulfate (E3′S) were the most abundant metabolites in all subjects. A high intra-individual correlation (r = 0.72, p < 0.001) between SREMs concentrations after single-dose and sustained supplementation was observed. The antioxidant capacity of plasma did not change in response to the intervention and was not correlated with any of the SREMs. Conclusion: The individual SREMs profile and concentrations after a 28-day supplementation are comparable to those after a single dose. |
| Databáze: | OpenAIRE |
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