Biofilm Formation and Acyl Homoserine Lactone Production in the Burkholderia cepacia Complex
| Autor: | B. Conway, Vicnays Venu, David P. Speert |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Cystic Fibrosis
Homoserine Burkholderia cepacia Polypropylenes Microbial Communities and Interactions Microbiology chemistry.chemical_compound 4-Butyrolactone Humans Molecular Biology chemistry.chemical_classification biology Biofilm food and beverages Genomovar Burkholderia Infections Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition biology.organism_classification Culture Media Quorum sensing Burkholderia cepacia complex Burkholderia chemistry Biochemistry Biofilms Lactone Signal Transduction |
| Zdroj: | Journal of Bacteriology. 184:5678-5685 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.184.20.5678-5685.2002 |
| Popis: | Acyl homoserine lactone (acyl-HSL)-mediated gene regulation has been shown to influence biofilm formation in one Burkholderia cepacia cystic fibrosis isolate, but it is not known whether this relationship is a consistent feature of the several genomic species that make up the B. cepacia complex (BCC). We screened strains belonging to genomovars I to V of the BCC for biofilm formation on an abiotic surface and for acyl-HSL synthesis. We determined that organisms from each of these genomovars were capable of biofilm formation. Similarly, acyl-HSL was synthesized by organisms from each of genomovars I to V, with most isolates producing octanoyl-HSL in greatest abundance. When biofilms were grown in Luria broth, acyl-HSL synthesis and biofilm formation appeared to be associated, but these phenotypes were independent when the biofilms were grown in basal salts containing citrate. Genomovar V strains synthesized the greatest quantities of acyl-HSL, and genomovar II and III-A strains elaborated the most abundant biofilms. Quorum sensing may play a role in BCC pathogenesis, but it may not regulate biofilm formation under all growth conditions. |
| Databáze: | OpenAIRE |
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