Heparin modulates adenine nucleotide hydrolysis by synaptosomes from cerebral cortex
| Autor: | João Rocha, A Mezzomo, Maria Rosa Chitolina Schetinger, F Michelot, F Falquembach |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Synaptic cleft
Potassium Chloride Divalent Cellular and Molecular Neuroscience Adenosine Triphosphate Sulfation Adenine nucleotide medicine Animals Magnesium Nucleotide Enzyme Inhibitors Rats Wistar 5'-Nucleotidase Cerebral Cortex chemistry.chemical_classification Synaptosome Adenine Nucleotides Heparin Hydrolysis Apyrase Dextran Sulfate Cell Biology Adenosine Adenosine Monophosphate Rats Adenosine Diphosphate Biochemistry chemistry Calcium Synaptosomes medicine.drug |
| Zdroj: | Neurochemistry International. 33:243-249 |
| ISSN: | 0197-0186 |
| DOI: | 10.1016/s0197-0186(98)00024-2 |
| Popis: | The modulatory effect of heparin and dextran sulfate 500,000 (sulfated polysaccharides) was studied on ATPDase and 5'-nucleotidase activities. These enzymes participate in the degradation of ATP and adenosine production at the synaptic cleft level. Nucleotide hydrolysis was inhibited by heparin and dextran sulfate 500,000. For ADP, the inhibition was more evident at low cation concentrations (0.15 mM Ca 2+ or Mg 2+ ), reaching a maximum of 75%. For ATP, the inhibitory effect was less prominent and independent of divalent cation concentration, reaching a maximum of 25%. For AMP, the inhibition observed was similar with either relatively high (I mM) or with low Mg 2+ concentrations tested (0.1 mM) and reached a maximum of 35%. K + did not change the inhibitory potency of sulfated polysaccharide suggesting that its effects were not exclusively related to charge interaction. These results suggest that heparin and possibly other naturally occurring sulfated polysaccharides may have a potential role as modulator of extracellular nucleotide hydrolysis in the synaptic cleft region. |
| Databáze: | OpenAIRE |
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