Subtraction hybridization - an approach to the isolation of genes differentially expressed in cancer and other biological-systems

Autor:	Cw Schweinfest, Ts Papas
Rok vydání:	2011
Předmět:	Genetics Cancer Research Cell type Subtraction hybridization Biology medicine.disease_cause genomic DNA Oncology Complementary DNA Nucleic acid medicine Carcinogenesis Gene Function (biology)
Zdroj:	International journal of oncology. 1(5)
ISSN:	1019-6439
Popis:	Subtraction hybridization allows for the isolation and identification of genes which are differentially expressed in different cell types. It allows for such isolation without any a priori knowledge of the sequence or function of the target gene. The only requirement is that there be a difference in the relative abundance of the nucleic acid (mRNA or genomic DNA) in the two cell types being compared. In contrast to differential hybridization, cDNA clones representing mRNAs of very low abundance (0.001% to 0.1%) can be discerned using subtraction hybridization. The concept, applications and techniques of performing subtraction hybridization is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b39883cd6ffb78818635e3484aad435d https://pubmed.ncbi.nlm.nih.gov/21584572 Zobrazit plný text záznamu