Direct Detection and Quantification of Transition Metal Ions in Human Atherosclerotic Plaques: Evidence for the Presence of Elevated Levels of Iron and Copper
| Autor: | Robyn A. Lindner, Nadina Stadler, Michael J. Davies |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Carotid Artery Diseases
Male Pathology medicine.medical_specialty Iron Metal ions in aqueous solution chemistry.chemical_element Mass spectrometry Mass Spectrometry law.invention Nuclear magnetic resonance Transition metal law medicine Humans Coronary Artery Bypass Mammary Arteries Spectroscopy Electron paramagnetic resonance Aorta Aged Aged 80 and over Endarterectomy Carotid Chemistry Electron Spin Resonance Spectroscopy Middle Aged Copper Carotid Arteries Cholesterol Radial Artery Female Inductively coupled plasma Tunica Intima Cardiology and Cardiovascular Medicine Ex vivo |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 24:949-954 |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/01.atv.0000124892.90999.cb |
| Popis: | Objective—The involvement of transition metals in atherosclerosis is controversial. Some epidemiological studies have reported a relationship between iron (Fe) and cardiovascular disease, whereas others have not. Experimental studies have reported elevated levels of iron and copper (Cu) in diseased human arteries but have often used methods that release metal ions from proteins.Methods and Results—In this study, we have used the minimally invasive technique of electron paramagnetic resonance (EPR) spectroscopy and inductively coupled plasma mass spectroscopy (ICPMS) to quantify iron and copper in ex vivo healthy human arteries and carotid lesions. The EPR spectra detected are characteristic of nonheme Fe(III) complexes. Statistically elevated levels of iron were detected in the intima of lesions compared with healthy controls (0.370 versus 0.022 nmol/mg tissue for EPR, 0.525 versus 0.168 nmol/mg tissue by ICPMS,PPConclusions—These data support the hypothesis that iron accumulates in human lesions and may contribute to disease progression. |
| Databáze: | OpenAIRE |
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