Site-specific molecular analysis of the bacteriota on worn spectacles
| Autor: | Melanie März, Focke Ziemssen, Severin Weis, Birgit Fritz, Markus Egert, Siegfried Wahl |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine Firmicutes Propionibacterium 030106 microbiology lcsh:Medicine Microbial communities medicine.disease_cause Microbiology Article Actinobacteria Young Adult 03 medical and health sciences RNA Ribosomal 16S medicine Humans lcsh:Science Bacterial phyla Skin Multidisciplinary Bacteria biology lcsh:R Bacteroidetes Biodiversity biology.organism_classification Eyeglasses 030104 developmental biology Fomites lcsh:Q Female Proteobacteria Staphylococcus |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Regularly touched surfaces are usually contaminated with microorganisms and might be considered as fomites. The same applies for spectacles, but only little is known about their microbial colonization. Previous cultivation-based analyses from our group revealed a bacterial load strongly dominated by staphylococci. To better account for aerotolerant anaerobes, slow growing and yet-uncultivated bacteria, we performed an optimized 16S rRNA gene sequencing approach targeting the V1-V3 region. 30 spectacles were swab-sampled at three sites, each (nosepads, glasses and earclips). We detected 5232 OTUs affiliated with 19 bacterial phyla and 665 genera. Actinobacteria (64%), Proteobacteria (22%), Firmicutes (7%) and Bacteroidetes (5%) were relatively most abundant. At genus level, 13 genera accounted for 84% of the total sequences of all spectacles, having a prevalence of more than 1% relative abundance. Propionibacterium (57%), Corynebacterium (5%), Staphylococcus (4%), Pseudomonas, Sphingomonas and Lawsonella (3%, each) were the dominant genera. Interestingly, bacterial diversity on the glasses was significantly higher compared to nosepads and earclips. Our study represents the first cultivation-independent study of the bacteriota of worn spectacles. Dominated by bacteria of mostly human skin and epithelia origin and clearly including potential pathogens, spectacles may play a role as fomites, especially in clinical environments. |
| Databáze: | OpenAIRE |
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